細胞外基質排列以及電場對韌帶細胞遷移的影響

Effects of ECM alignment on migration of ligament fibroblast with applied DC EF

游仕帆(Shih-Fan Yu)

指導教授 ： 趙本秀

國立臺灣大學/工學院/醫學工程學研究所/碩士(2010年)

https://doi.org/10.6342/NTU.2010.02143

摘要

十字韌帶具有穩定關節功用，因癒合能力極小，而導致日後關節疼痛。本實驗利用外加電場促進十字韌帶癒合。在正常生理上細胞遷移扮演重要角色，包括傷口癒合以及型態發生。使用豬膝關節韌帶細胞外加電場，研究細胞在自然的基質遷移及纖維排列走向影響。我們發現纖維走向和電場會影響到細胞的移動。細胞型態在沒有電場時方向性是呈現任意走向，纖維排列與電場方向垂直細胞型態呈現圓形。我們進一步利用排列膠原蛋白模擬基質的結構，膠原蛋白纖維和電場垂直時會降低細胞的遷移。因此在纖維排列上確實會造成細胞移動的影響。

關鍵字

電刺激 ； 韌帶 ； 膠原纖維排列

並列摘要

Electric fields (EFs) induce and enhance cell migration in various tissues. We applied electric field to enhance the migration of the ligament fibroblasts on its native matrix, and the alignment of the collagen fibers also affects the migration of the fibroblasts. Cell migration plays an important role in normal physiological activities, including wound healing and morphogenesis. Cruciate ligament is mainly used to stabilize the knee joint, and ligaments are the most common injury sites. When subject to applied EF, ligament fibroblasts enhance migration along their native matrix when fibers were oriented parallel to the direction of the field. We further simplify ligament structure to use collagen alignment to test structure effect. When collagen fibers were oriented perpendicular to the direction of the field, cells migrate slowly. Therefore, collagen fiber alignment effects cells migration.

並列關鍵字

electric field ； knee ligament ； collagen fiber alignment

參考文獻

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