- 學位論文
多功能聚對二甲苯:奈米尺度靈活與專一性之表面控制
Multifunctional Poly-para-xylylenes: Flexibility and Specificity of Controlled Biointerfaces in Nano-Scaled Dimension
摘要
奈米科技由於其高表面積的特性，在生物科學、醫學和工業等方面都扮演極其重要的角色。而近年來，奈米科技的研究正逐步向多組分系統進行發展，結合新型多功能性鍍膜的表面改質技術於奈米科技，可以使相關的應用得到重大的突破。本研究使用聚對二甲苯高分子製造出兩種多功能性奈米粒子，達到專一與靈活的表面反應性，以期能在複雜的生物系統中成功結合各種生物分子。我們使用化學氣相沉積技術與奈米沉澱法製造出的奈米粒子，可以進行azide-alkyne環化反應, thiol-maleimide點擊反應和原子轉移自由基聚合反應來達到專一性鍵結，或是利用NHS-ester-amine 偶聯反應和光聚合交聯反應來達到表面的靈活性控制。實驗中，除了透過各種分析儀器來驗證奈米粒子的平均粒徑、外觀形態及表面化學特性外，我們也將其與螢光和聚甲基丙烯酸乙二醇酯(PEGMA)分子做結合，以確認其反應性。最後，多功能性奈米粒子做為標靶抗癌藥物的載體，鍵結上太平洋紫杉醇(paclitaxel)及葉酸(folic acid)分子，並實際進行海拉細胞(HeLa cell)的生長抑制測試。
並列摘要
Because of high surface to volume ratio, nanotechnology has played an important role in the biological science, medicine and industry. Recently, the development of nanotechnology has evolved into an era of multi-component systems. By introducing novel multifunctional coating to surface modification in nano-scaled dimension, applications of nanotechnology may make an important breakthrough. In this study, two kinds of multifunctional nanocolloids based on poly-para-xylylene were created to achieve specific and flexible reactivity with numerous biomolecules in complicated biological systems. Fabricated by chemical vapor deposition and nanoprecipitation methods, these nanoparticles could possess specificity through azide-alkyne cycloaddition, maleimide click reaction and surface-initiated atom transfer radical polymerization or flexibility through NHS-ester-amine coupling reaction and the photochemcially induced crosslinking reaction. Average sizes, morphologies, and surface chemistry of nanoparticles were characterized with a series of analysis. In addition, conjugations of fluorescent probes and poly(ethylene glycol) methacrylate (PEGMA) were also accomplished to confirm reactivity. Finally, application as a targeted drug carrier by immobilized with paclitaxel and folic acid molecules was demonstrated to inhibit growth of HeLa cancer cell.