口腔鏈球菌與人類口腔癌細胞，白血球，及癌症相關纖維母細胞之交互作用

Interactions of Oral Streptococci with Human Oral Cancer Cells, Leukocytes and Cancer-Associated Fibroblasts

蔡旻潔(Min-Jie Tsai)

指導教授 ： 賈景山

國立臺灣大學/醫學院/微生物學研究所/碩士(2010年)

https://doi.org/10.6342/NTU.2010.10535

摘要

無資料

關鍵字

口腔癌 ； 口腔共生鏈球菌 ； 單核球 ； 癌症相關纖維母細胞 ； 發炎反應

並列摘要

In the particular microenvironment of oral squamous cell carcinoma (OSCC), the orchestra of cancer cells, infiltrating immune cells, certain bacteria (such as Streptococcus anginosus), and stromal fibroblasts (cancer-associated fibroblasts, CAFs) play a symphony of cancer-related inflammation. In oral cancer, the role of CAFs in modulating the cancer-assoicated inflammation remains poorly understood. The CAFs isolated and cultured from oral cancer tissues are characterized with human fibroblast markers. Recombinant human IL-1β activates CAFs in a dose-dependent manner, and the production of IL-6, IL-8 and MCP-1 in supernatant accumulates as the stimulation time increases. Recombinant IL-1β-activated CAFs express increased levels of proinflammtory cytokines (such as IL-6), and chemokines which are associated with cancer progression, and recruitment of monocytes/macrophages (including IL-8, MCP-1, MCP-3, and GM-CSF). Stimulation of OSCC cells and peripheral blood mononuclear cells with S. anginosus induces the factors which activate CAFs in an IL-1-dependent manner. Conditioned media of CAF promote migration of human monocytes, and IL-1β activation enhances the chemotactic effect. Taken together, both the interaction with invaded streptococci as the exogenous stimuli for immune cells and the endogenous property of OSCC cells lead to IL-1 production. Activation of stromal fibroblasts by IL-1 may give rise to tumor-promoting inflammation in oral cancer microenvironment.

並列關鍵字

oral cancer ； oral commensal streptococci ； monocyte ； cancer-associated fibroblast ； inflammation

參考文獻

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