地衣桿菌發酵產物對貓慢性下痢與腸道微生物群之影響

The Effects of Bacillus licheniformis-fermented Products on Feline Chronic Diarrhea and Intestinal Microbiota

李庭瑋(Ting-Wei Lee)

指導教授 ： 張惠雯

共同指導教授 ： 武敬和(Ching-Ho Wu)

國立臺灣大學/獸醫專業學院/分子暨比較病理生物學研究所/碩士(2021年)

https://doi.org/10.6342/NTU202104050

摘要

地衣桿菌發酵產物（Bacillus licheniformis-fermented products, BLFP）作為益生菌，已被證實具抗細菌、抗病毒和抗發炎的能力。此研究目的在於使用次世代定序比較同一飼主飼養的慢性下痢貓隻（八隻）和正常貓隻（四隻）的腸道微生物群，及評估服用地衣桿菌發酵產物對於慢性下痢貓隻的臨床症狀及腸道微生物群改變之影響。在服用BLFP的八隻慢性下痢貓隻中，有六隻貓呈現臨床上的改善，其中有兩隻糞便狀況轉為正常。下痢貓隻的厚壁菌門/擬桿菌門（Firmicutes/Bacteroidetes）的比值高於健康貓隻，此比例在有臨床改善的下痢貓隻也高於無改善的下痢貓隻。下痢貓隻無論是否有無服用BLFP的擬桿菌門（Bacteroidetes）和擬桿菌綱（Bacteroidia）皆顯著低於正常貓隻。服用BLFP後的下痢貓隻相對於服用前有顯著較高的Blautia spp.、Ruminococcus torques和Ruminococcus gnavus，此三種菌屬於梭狀桿菌屬第十四群菌，且報告顯著具益於維持腸道健康功能。此外，在服用BLFP後的下痢貓隻腸道中產氣莢膜梭菌（Clostridium perfringens）有顯著減少之情形。整體來說，服用BLFP在下痢貓隻具有改善臨床症狀和腸道微生物叢的效益。

關鍵字

地衣桿菌發酵產物 ； 益生菌 ； 腸道微生物群 ； 貓慢性下痢 ； 次世代定序

並列摘要

Bacillus licheniformis-fermented products (BLFP) is one of probiotics proven to improve growth performance with antibacterial, antiviral, and anti-inflammatory abilities. The aims of this study were to compare the fecal microbiota of cats with chronic diarrhea (n=8) with that of healthy cats (n=4) from the same household by next generation sequencing (NGS) and the evaluate effectiveness of oral administration of BLFP on relieving the clinical sings and altering the intestinal microbiota in diarrheal cats. Six out of the 8 diarrheal cats showed clinical improvement on fecal condition after BLFP administration for 7 days. Among the 6 cats, the stool condition became normal in 2 cats. A higher Firmicutes/Bacteroidetes ratio was noted in the feces of diarrheal cats as compared with those in the control healthy cats and in the diarrheal cats with clinical improvement after having BLFP as compared with these in the diarrheal cats without clinical improvement. The phylum Bacteroidetes and class Bacteroidia decreased significantly in the diarrheal cats regardless of the administration of BLFP. Blautia spp., Ruminococcus torques, and Ruminococcus gnavus, which belong to the Clostridium cluster XIVa and have been reported beneficial to the intestinal health increased significantly in the feces after BLFP treatment. Furthermore, a significant decrease of Clostridium perfringens was also noted in diarrheal cats after BLFP administration. Overall, BLFP could be a potential probiotic to relieve GI signs and improve fecal microbiota in chronic diarrheal cats.

並列關鍵字

Bacillus licheniformis-fermented products ； probiotics ； fecal microbiota ； feline chronic diarrhea ； next generation sequencing

參考文獻

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