- 學位論文
Serratia marcescens TKU011 發酵生產靈菌紅素之研究
Studies on the production of prodigiosin by Serratia marcescens TKU011 fermentation
摘要
本研究主要探討篩選自台灣中部土壤之靈菌紅素(prodigiosin)生產菌Serratia marcescens TKU011，生產靈菌紅素之液態發酵條件。研究結果發現，充填0.1% α-chitin、0.06% casein、0.005%K2HPO4、0.01% CaSO4˙2H2O之10毫升液態培養基（pH 6.15）的50mL錐形瓶，於25℃震盪培養箱（150 rpm）連續培養兩天，可得較高靈菌紅素生產量（6.25 μM）。本實驗探討所得靈菌紅素生產條件，比起過去使用烏賊軟骨作為主要碳/氮源所得靈菌紅素產量（3.4 μM）高出1.84倍。分離所得靈菌紅素之分子結構，經由1H-NMR(300MHz)、MASS (MALDI-TOF)、FT-IR等儀器分析獲得確證。
並列摘要
This study focused on the selecting of Serratia marcescens TKU011, a strain of prodigiosin-producing bacteria from the soil of middle Taiwan, to produce liquid fermentation conditions for the production of prodigiosin. The results showed that a 50 mL Erlenmeyer flask fill with 10 mL liquid medium (pH 6.15) containing 0.1% α-chitin, 0.06% casein, 0.005% K2HPO4, 0.01% CaSO4˙2H2O are shaken at 25℃(150 rpm) continuous culture for two days yielded higher prodigiosin production (6.25 μM). In this experiment, the production conditions of obtained prodigiosin are 1.84 times higher than the production of squid pen powder (3.4 μM) which is used as the main carbon / nitrogen in the past. The molecular structure of the obtain prodigiosin is separated and confirmed by 1H-NMR (300MHz), MASS (MALDI-TOF), FT-IR and other instruments.