- 學位論文
不同矽基底骨水泥之理化性質與生物活性分析
Analysis of the physicochemical properties and bioactivity of different silica-based bone cements
摘要
矽酸鈣因其成骨特性已被證明在生物醫學應用上具有極大的潛力，人體中存在著不同的微量元素，已有研究證實其中某些元素在細胞增殖過程中發揮著重要作用，例如:鎂與鍶。鎂可以刺激成骨細胞增殖，鎂缺乏會導致骨骼生長受損、骨小梁損失增加和骨吸收，鍶可以藉由同時刺激成骨細胞活性與抑制破骨細胞的吸收達到促進骨的再生，因此常用於治療骨質疏鬆症。 本研究使用溶膠凝膠法與水熱法製備鎂、鈣、鍶之矽基粉末，評估使用兩種不同合成方式所得到的粉末其物理化學性質是否相同，並將粉末與水混合後進行相結構、徑向拉伸強度、硬化時間等分析，以及進行pH 5、 pH 7.4兩種不同酸鹼值之體外浸泡測試，來評估不同矽基骨水泥其生物活性。結果顯示使用兩種不同合成方式所得到的鎂、鈣、鍶之矽基粉末分別由Mg2SiO4、β-Ca2SiO4、Sr2SiO4所組成。粉末與水混合後唯獨鈣的組別由β-Ca2SiO4相轉變為方解石(CaCO3)相。含鎂與鈣之矽基骨水泥硬化時間皆落在20分鐘左右，而鍶的組別則長達30分鐘以上。經由1個月的體外浸泡後，唯獨含鈣之矽基骨水泥表面形成磷灰石層。徑向拉伸強度所有組別在浸泡後皆高於浸泡前。
並列摘要
Calcium silicate has been shown to have great potential in biomedical applications due to its osteogenic properties. Different trace elements exist in the human body, and studies have confirmed that some of these elements play an important role in the process of cell proliferation, such as Magnesium and Strontium. Magnesium can stimulate osteoblast proliferation, deficiency can lead to impaired bone growth, increased trabecular bone loss, and bone resorption. Strontium can promote bone regeneration by simultaneously stimulating osteoblast activity and inhibiting osteoclast resorption, Commonly used to treat osteoporosis. In this study, the sol-gel method and hydrothermal method were used to prepare containing magnesium, calcium, and strontium silicon-based powders, to evaluate whether the physical and chemical properties of the powders obtained by two different synthesis methods are the same, and the powders were mixed with water to carry out the phase structure , diametral tensile strength, setting time, etc., and in vitro immersion tests to evaluate the biological activity of different silica-based bone cements. The results show that the silicon-based powders of magnesium, calcium, and strontium obtained by two different synthesis methods are composed of Mg2SiO4, β-Ca2SiO4, and Sr2SiO4, respectively. After the powder is mixed with water, only the calcium group changes from β-Ca2SiO4 phase to calcite (CaCO3) phase. The setting time of the silica-based bone cements containing magnesium and calcium is about 20 minutes, while the setting time of the strontium group is more than 30 minutes. After 1 month of in vitro immersion, only the calcium-containing silica-based bone cement formed an apatite layer. The diametral tensile strength of all groups after soaking is higher than before soaking.