- 學位論文
探討ZO-1在鼻息肉組織中的表現與嗜酸性球浸潤的關聯性
Investigating the correlation between nasal polyp ZO-1 expression and eosinophil infiltration
摘要
在許多研究中發現，在慢性鼻竇炎患者中的鼻竇組織，絕大多數都有嗜酸性球浸潤的情形，這樣的情況中又可分為黴菌感染和鼻息肉患者兩類，因此鼻息肉的出現可能與嗜酸性球浸潤有某種關係。另外，雖然有些研究有提出鼻息肉的形成可能與黏附因子有關，卻極少提到嗜酸性球浸潤是否也會影響鼻息肉裡細胞黏附因子表現的改變。本研究將病理組織分為三組，分別為第一組: 未過敏控制組，具低度嗜酸性球浸潤；第二組: 慢性鼻竇炎有黴菌感染，具高度嗜酸性球浸潤；以及第三組，慢性鼻竇炎有鼻息肉，具高度嗜酸性球浸潤。我們利用病理常見的H＆E染色觀察嗜酸性球的浸潤情形並以PAS染色來觀察黏液在鼻竇組織的狀況，另外以ZO-1的IHC染色觀察細胞黏附因子的變化。研究結果發現，在H＆E染色中第三組比起另外兩組有大量的嗜酸性球浸潤，第三組在PAS 染色中也發現大量的黏液。第三組的ZO-1相較第二組和第一組表現量也較多，在E-cadherin上這三組是沒有太大的差異。 結果發現，ZO-1的表現量雖然在鼻息肉中會提高，但ZO-1與鼻息肉之間的關聯性仍需進一步的研究。此外，ZO-1表現量的變化與嗜酸性球浸潤的多寡並無顯著關聯。此一變化可能是嗜酸性球浸潤到鼻息肉之後所造成，至於ZO-1及其他細胞黏附因子在鼻息肉的變化機制則需更進一步的研究。
並列摘要
Background: Previous studies have found that nasal tissues in most patients with chronic sinusitis exhibit eosinophil infiltration, and generally these patients show fungal infections and nasal polyps. Therefore, it is suggested that nasal polyps are associated with eosinophil infiltration. Although some studies have suggested that nasal polyps are linked to dysregulation of cell adhesion molecules (CAMs), the role of infiltrate eosinophils in expression of CAMs in nasal polys remains sketchy. Aims: This study was aimed to investigate the correlation between expression of CAMs and eosinophil infiltration in nasal polyps. Methods: Nasal polyps were collected from healthy subjects (control group) and chronic sinusitis patients with fungi infection (FI group) or eosinophil infiltration (EI group). Eosinophil infiltration and mucus production were demonstrated by using hematoxylin and eosin (H&E) staining and periodic acid staining, respectively. CAM expression was determined by immunohistochemical (IHC) staining using specific antibodies. Results: Our results showed that the highest eosinophil infiltration was observed in EI group among the three groups. In addition, mucus level in EI group was higher than the other group. For CAM expression, ZO-1 expression in EI group was significantly higher than that in the other groups (P<0.05). On the contrary, E-cadherin expression showed insignificantly altered among the three groups. Conclusions: Our findings reveal that ZO-1 expression in nasal polys with eosinophil infiltration is higher that with fungi infection. However, whether the higher ZO-1 expression in nasal polyps is associated with eosinophil infiltration needs further investigation.