- 學位論文
輔酶Q10濃度與氧化壓力、抗氧化酵素活性及冠狀動脈心臟病危險對比值相關性之探討
The relationship between coenzyme Q10 concentration, oxidative stress, antioxidant enzyme activities and the odds ratio of coronary artery disease
摘要
許多研究發現冠狀動脈心臟病（Coronary artery disease，CAD）與氧化壓力有關。輔酶Q10為粒線體膜上的內生性脂溶性抗氧化劑。本研究目的為探討血漿輔酶Q10濃度與氧化壓力及抗氧化酵素活性之相關性，並了解血漿輔酶Q10濃度對罹患CAD危險對比值之影響。CAD受試者利用心導管檢查結果至少一條冠狀動脈狹窄程度大於50%以上者為病例組（n=51）。健康受試者經體檢查資料，其血液生化值於正常範圍內，做為對照組（n=102）。測量受試者之體位測量、血液生化值檢驗、血漿輔酶Q10、丙二醛（malondialdehyde，MDA）濃度及抗氧化酵素活性[過氧化氫酶，catalase（CAT）；超氧歧化酶，superoxide dismutase（SOD）；麩胱甘肽過氧化酶，glutathione peroxidase（GPx）]之測量。利用問卷調查了解受試者日常生活習慣；以二十四小時回憶法了解受試者營養素攝取量。結果顯示，病例組的年齡、收縮壓、身體質量指數、腰臀比、血液生化值、男性人數及運動人數比例顯著高於對照組；而高密度脂蛋白、血漿輔酶Q10濃度則顯著低於對照組。抗氧化酵素方面，病例組的CAT及GPx活性顯著低於對照組；但SOD活性顯著高於對照組。血漿輔酶Q10濃度與CAT或GPx呈顯著正相關，與SOD活性呈顯著負相關；輔酶Q10與MDA則無相關。當受試者有較高濃度的血漿輔酶Q10（≧0.52 μM）可顯著降低罹患CAD之風險。本研究結果支持血漿輔酶Q10是一保護心臟之重要抗氧化劑。
並列摘要
A higher oxidative stress may contribute to the pathogenesis of coronary artery disease (CAD). Coenzyme Q10 is recognized as an endogenous lipid-soluble antioxidant located in the mitochondrial membrane. The purpose of this study was to investigate the relationship between coenzyme Q10 concentration and lipid peroxidation, antioxidant enzymes activities and the risk of CAD. Patients who were identified by cardiac catheterization as having at least 50% stenosis of one major coronary artery were assigned to the case group (n=51). The control group (n=102) comprised healthy individuals with normal blood biochemical values. Anthropometry, hematologic parameters, plasma coenzyme Q10, malondialdehyde (MDA) concentrations and antioxidant enzymes activities [catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx)] were measured. A questionnaire was used to obtain subjects’ smoking, drinking and exercise habits. A 24-h diet recall was used to understand the nutrients intakes of subjects. Subjects in the case group had significantly higher values for age, systolic blood pressure, body mass index, waist to hip ratio, hematological entities, the numbers of males, exercise habit and lower levels of high density lipoprotein-cholesterol and plasma coenzyme Q10 than the control group. In respects of antioxidant enzymes, subjects in the case group had significantly lower CAT and GPx activities but higher SOD activities than the control group. The plasma coenzyme Q10 concentration was positively correlated with CAT and GPx activities but negatively correlated with SOD activities. There was no significant correlation between the levels of coenzyme Q10 and MDA. A higher level of plasma coenzyme Q10 (（≧0.52 μM) was significantly associated with reducing the risk of CAD. Our results support the potential cardioprotective impact of coenzyme Q10.