- 學位論文
黃原膠/結冷膠微粒之製備及應用
Preparation and application of xanthan gum/ gellan gum microspheres
摘要
在台灣，腎癌並不列於十大癌症死因，但是也不容忽視，一般都以手術切除作為治療方法，而腎動脈栓塞則是另外一種治療法，但是由於容易造免疫或發炎等不良反應，因此不常使用，但是如果能挑選適合的基材作為栓塞，那麼或許就能在治療上多一項選擇。因此研究黃原膠/結冷膠再包覆金聖草素後的效用及分析微粒的各項數值。在黃原膠/結冷膠/金聖草素微粒的體外釋放，不同的粒徑釋放的累積濃度沒有顯著差異，而其釋放的動力學模式符合Higuchi及Korsmeyer-peppas兩種模式。用不同濃度包覆有金聖草素的微粒體外釋放，做細胞存活測試，細胞存活率都有明顯的下降。在流式細胞儀也觀測到含有金聖草素的微粒有細胞凋亡的現象。栓塞於腎動脈後，也可看到腎臟有稍微的變小。而根據上述結果，本研究認為將黃原膠/結冷膠/金聖草素運用在栓塞腎臟癌是具有潛力的。
並列摘要
In Taiwan, renal cancer is not listed among the top ten causes of cancer death, but it cannot be ignored. Generally, surgical resection is used as the treatment, while renal artery embolization is another method. Therefore, it is not often used, the reason is to select the suitable substrate for the embolization is difficult. But if we can find that, it may be possible to have an additional choice in treatment. The effect of xanthan gum/ gellan gum after being coated with chrysoeriol were analyzed and calculated. The in vitro release of xanthan gum/ gellan gum/ chrysoeriol microspheres, there was no significant difference in the cumulative concentrations released with different particle sizes, and the pharmacokinetics were consistent with Higuchi and Korsmeyer-peppas models. Cell viability assay was carried out with different concentrations of microspheres coated with chrysoeriol released in vitro, and the cell viability decreased significantly on the second day. Apoptosis was also observed in the microspheres containing chrysoeriol by flow cytometry. After embolization in the renal artery, the kidney can also be seen to be slightly smaller. Based on the above results, this study believes that the application of xanthan gum/ gellan gum/ chrysoeriol microspheres in embolization of renal cancer has potential.