- 學位論文
探討超氧歧化酶1基因多樣性與急性心肌梗塞的相關性
The association of Superoxide Dismutase 1 polymorphism and acute myocardial infarction.
摘要
在世界上多數已開發國家中，冠狀動脈心臟病為主要的死因之一，在西元2004年從我國國民十大死因之第四位提升至第二位，其中最主要的疾病，急性心肌梗塞，則為台灣國人重要的死因之一。關於其危險因子的探討則著重在如吸菸、高血壓與糖尿病之有無等傳統危險因子的討論居多；然而，以上各種經研究證實的傳統危險因子並無法完全解釋急性心肌梗塞發生的原因。 當身體內的氧化物質與抗氧化機制無法達到平衡狀態時，就會導致氧化壓力的產生，而氧化壓力又與許多疾病有相關聯，例如：心血管疾病、癌症。SOD1 (copper/zinc superoxide dismutase or superixde dismutase 1)屬抗氧化物，其生理機轉是將O2轉變成H2O2，占所有SODs的90%，並廣泛存在細胞質中。 本研究主要探討台灣地區SOD1與急性心肌梗塞之相關性。實驗人數共65名，利用分析PCR-HhaI-digested DNA片段的方法偵測。研究結果發現SOD1(superoxide dismutase 1)在C/A基因多型性與對照組無顯著差異；因此我們認為台灣地區SOD1 C/A基因多型性與心肌梗塞好發性之間無相關性。
關鍵字
超氧歧化酶基因多樣性並列摘要
Coronary heart disease (CHD) is one of the predominant causes of disease-related death and disability in all industrialized countries. Heart disease was the second leading death in Taiwan in 2004. Acute myocardial infarction (AMI) is a major CHD in Taiwan. Among risk factors identified by epidemiological studies, smoking, hypertension, and diabetes mellitus are considered the most important predictors for the risk of AMI at the individual level. Oxidative stress, which is due to primarily imbalance between reactive oxygen sprcies (ROS) and antioxidants in favor of the former, has been a focus of research for many years. Copper-zinc superoxide dismutase (SOD1) is widely distributed and comprises 90% of the total superoxide dismutase (SOD), catalyzes the conversion of superoxide to hydrogen peroxide. The aim of this study was to examine whether polymorphism of SOD1 gene is association with the risk of AMI. We have performed a case-control study from 65patients. SOD1 gene polymorphism was determined by analyzing HhaI-digested DNA fragment obtained by PCR. An association of the SOD1 C/A genetype of control to the AMI onset was not found. In conclusion, our result suggested that SOD1 polymorphism was not associated with AMI among Taiwan with convestional cardiovascular disease risk factor.