- 學位論文
臺灣山香圓成分及其骨質新生活性探討
The constituents and their osteogenesis activities from Turpinia formosana Nakai
摘要
臺灣山香圓 (Turpinia formosana Nakai) 為省沽油科(Staphyleaceae) 山香圓屬 (Turpinia) 之臺灣特有種植物，其為遍產於海拔1500 公尺以下闊葉林內之常綠喬木。本研究乃採集大量臺灣山香圓的葉部，以95% 酒精萃取並取其乾燥後粗萃物進行分配萃取，部分劃分後再以多種管柱層析方式加以進行成分分離，得到五個化合物。其中自乙酸乙酯層中得到一新的鞣花酸衍生物，經由理化及光譜數據分析鑑定其鞣花酸衍生物結構為3,3′-di-O-methylellagic acid-4-O-α-L-arabinofuranoside (1)，為未發表過之新化合物。其餘四個化合物，經由理化及光譜相關數據分析以及文獻比對後，確認其四個化合物之結構為gentisic acid 5-O-β-D-(6’-O-galloyl)glucopyranoside (2)，strictinin (3)，casuarinin (4)，及casuariin (5)。在活性評估部分，以自臺灣山香圓分離而得的五個化合物，利用人類造骨細胞 (human osteoblasts) 進行相關活性試驗：於MTT細胞存活率試驗中，發現3,3′-di-O-methylellagic acid-4-O-α-L-arabinofuranoside (1) 以及gentisic acid 5-O-β-D-(6’-O-galloyl)glucopyranoside (2) 細胞存活率> 80%；對促進鹼性磷酸酶表現之試驗中，發現3, 3′-di-O-methylellagic acid-4-O-α-L-arabinofuranoside (1) 有較顯著的促進效果；進一步在礦化染色試驗中，發現3,3′-di-O-methylellagic acid-4-O-α-L-arabinofuranoside (1)及gentisic acid 5-O-β-D-(6’-O-galloyl)glucopyranoside (2) 對於礦化染色表現均有顯著促進效果，有助於將來骨質新生方面之應用。
並列摘要
Turpinia formosana Nakai (Staphyleaceae) is an endemic plant which is widely found in Taiwanese mountains at medium elevations. The outward appearance of T. formosana is a small evergreen tree. In this present study, the leaves of T. formosana were extracted by 95% ethanol, and then partitioned with a sequence of solvents. The EtOAc layer and n-BuOH layer were passed by a series of chromatography. The structures of isolated compounds were identified by various physical and spectroscopic characterizations. There were five compounds isolated from the leaves of T. formosana , including one new ellagic acid derivative from EtOAc layer : 3,3′-di-O-methylellagic acid-4-O-α-L-arabinofuranoside (1) and four known compounds from n-BuOH layer are : gentisic acid 5-O-β-D-(6’-O-galloyl)glucopyranoside (2), strictinin (3), casuarinin (4), and casuariin (5). For the cell viability, 3,3′-di-O-methylellagic acid-4-O-α-L-arabinofuranoside and gentisic acid 5-O-β-D-(6’-O-galloyl)glucopyranoside had no toxicity to HOb (cell viability > 80%). In the HOb osteogenic activity, 3, 3′-di-O-methylellagic acid-4-O-α-L-arabinofuranoside showed potential in the expression of ALP level. In the mineralization of 3,3′-di-O-methylellagic acid-4-O-α-L-arabinofuranoside and gentisic acid 5-O-β-D-(6’-O-galloyl)glucopyranoside, the results showed potential in the expression of mineralization level.