- 學位論文
牛樟芝用於改善骨質疏鬆小鼠骨流失現象及肺癌骨轉移之研究
Antrodia camphorata treatment rescues bone loss for decreasing lung cancer bone metastasis in osteoporotic mice
摘要
目前臨床上骨質疏鬆與骨轉移之病人最常使用雙磷酸鹽類藥物來抑制骨吸收與骨轉移，但該藥物會導致骨頭異常增生以及心血管疾病之潛在風險。故本研究欲使用牛樟芝酒精萃取物做為取代雙磷酸鹽類之藥物，以促進骨質疏鬆老鼠之骨再生及減少肺癌骨轉移之發生。研究主要分為兩個階段，第一階段為觀察中草藥牛樟芝促進骨母前驅細胞骨分化及減緩骨質疏鬆症之骨流失現象；第二階段則探討中草藥牛樟芝萃取物對骨質疏鬆小鼠之肺癌骨轉移的影響。結果證實骨母前驅細胞處理牛樟芝酒精萃取物後具較高的骨分化活性；骨質疏鬆老鼠餵食牛樟芝酒精萃取物後，骨質密度、骨體積及骨小樑皆有顯著增加；利用非侵入式活體影像分析系統證實牛樟芝具減少肺癌細胞骨轉移之能力。本研究結果顯示牛樟芝酒精萃取物具促進骨質疏鬆老鼠骨生成之作用及減少肺癌骨轉移之功效，並希冀牛樟芝酒精萃取物能成為治療肺癌骨轉移之新選擇。
並列摘要
Currently, the patients of osteoporosis and bone metastasis usually used bisphosphonates to inhibit bone resorption and suppress bone metastasis. But bisphosphonates could have both bone and cardiovascular side effects (e.g. bone abnormalities). In this study, we would use Antrodia camphorata alcohol extract (ACAE) as a good alternative drug to promote bone formation for decreasing bone metastasis in animal models. The specific aims of this study are (1) The potential use of ACAE to achieve preosteoblastic differentiation and rescue bone loss in osteoporosis therapy. (2) Using the ACAE to observe the effect of osteogenesis on preclinical model of cancer metastasis. Using several in vivo and in vitro assays, we have demonstrated that the pre-osteoblast cells treated with ACAE demonstrated higher differentiation activity than control group. Most importantly, in osteoporotic mice, ACAE treatment resulted in significantly increased in bone mineral density, bone volume and trabecular number. And then, we used In Vivo Imaging System (IVIS) to observe the ACAE could decreased bone metastasis. These results demonstrated that ACAE treatment could promote osteogenesis and decrease bone metastasis. Data presented here demonstrate that ACAE treatment may offer a novel therapeutic option for decreasing tumor burden and rescue bone loss in lung cancer to bone metastasis.