摘要
「安胎飲」、「固胎煎」、「所以載丸」、「泰山盤石散」、「援土固胎湯」，為臨床 常用中藥安胎方劑。依傳統煎煮方式用水抽取得各方劑之水萃取物。進行對離體大白鼠子 宮平滑肌作用評估後，產生劑量依存性的鬆弛作用，由其IC50得知其活性依序為泰山磐石 散(1.04±0.14mg/ml)＞安胎飲(1.25±0.25mg/ml)＞所以載丸(1.35±0.21mg/ml)＞固胎 煎(1.54±0.34mg/ml)＞援土固胎湯(1.78±0.36mg/ml)。結果「泰山盤石散」有明顯且穩 定的抑制作用。「泰山盤石散」出典於《景岳全書》，其組成生藥材為熟地Rehmannia gl utinosa Liboschitz var. hueichingensis Chao et Shih、白芍Paeonia lactiflora Pa llas、白朮Atractylodes ovata De Candolle、人參Panax ginseng C.A Meyer、當歸Ang elica sinensis Diels、川芎Ligusticum Chuanxiong Hort、續斷Dipsacus japonicus M iquel、黃芩Scutellaria baicalensis Georgi、黃耆Astragalus mongholicus Bunge、 炙甘草Glycyrrhiza uralensis Fischer et DC.、砂仁Amomum xanthioides Wallich、 糯米Oryza sativa L.，為婦科臨床常用於主治胎元不固、胎動不安之方。為了進一步探 討其作用機轉，乃先加入泰山磐石散之水萃取物於懸吊有離體大白鼠子宮平滑肌之臟浴器 中，再以累積方式分別加入投予子宮收縮劑oxytocin、acetylcholine(ACh)及Prostaglan dine F2α(PGF2α)。由各藥物之劑量-反應曲線分析，顯示泰山磐石散之水萃取物(0.5-2 .0mg/ml)對oxytocin 、ACh、 PGF2α所引起收縮呈現有意義地抑制，證實其屬非競爭性 拮抗。在高鉀（56.3mM）溶液中，泰山磐石散之水萃取物能抑制因高濃度鉀離子引起的去 極化收縮，同時也對oxytocin誘生自發性收縮有抑制，而又在Ca2+-free溶液中，使oxyto cin所引起之收縮產生鬆弛。此表示泰山磐石散之水萃取物能抑制子宮平滑肌上的Voltage operated calcium channels(VOCs)及/或Receptor operated calcium channels(ROCs) 。綜合上述結果，泰山磐石散之安胎效用主要是抑制子宮平滑肌收縮，其生理性作用機轉 為與oxytocin 、ACh、 PGF2α有非競爭性拮抗，且對細胞外鈣離子流入及細胞內鈣離子 釋放都有抑制作用。
關鍵字
泰山磐眼石飲並列摘要
「An-Tai In」、「Gu-Tai Jian」、「Suo-I-Ji Wan」、「Tai-Shan-Pan-Shi San」an d「Sho-Tu-Gu-Tai Tang」, are clinical tocolytic agents on used commonly Chines e gynecology medicinal prescription. We applied the water extracted component of these tocolytic agents to test their muscle dilation effect on the uterus s mooth muscle. We demonstrated a dose -dependent relaxation of tocolygic agents on the isolated uterus smooth muscle. Their IC50 were Tai-Shan-Pan-Shi San (1 .04±0.14mg/ml)，An-Tai In (1.25±0.25mg/ml)，Suo-I-Ji Wan (1.35±0.21 mg/ ml) ，Gu-Tai Jian (1.54±0.34mg/ml) and Sho-Tu-Gu-Tai Tang (1.78±0.36mg/ml)，resp ectives. We condoned that Tai-Shan-Pan-Shi San was significantly inhibited the uterus smooth muscle.Tai-Shan-Pan-Shi San (TPS)was oringally descended in Jin - Ye - Chuan-Shu. It was composed of Rehmannia glutinosa Liboschitz var. huei chingensis Chao et Shih、Paeonia lactiflora Pallas、Atractylodes ovata De Cand olle、Panax ginseng C.A Meyer、Angelica sinensis Diels、Ligusticum Chuanxiong Hort、Dipsacus japonicus Miquel、Scutellaria baicalensis Georgi、Astragalus mo ngholicus Bunge、Glycyrrhiza uralensis Fischer et DC.、Amomum xanthioides Wall ich and Oryza sativa L..It has been used to treat the abortion or premature la bor. To study the mechanism of its action , the different concentrations of TP S were administered into organ bath with the isolated rat uterus smooth muscle . Then a cumulative concentrations of oxytocin, acetylcholine (ACh), and prost aglandine F2α(PGF2α), were administered . Based on the studies of dose-respo nse relationship , we found that that TPS (0.5-2.0mg/ml) significantly inhibit ed the oxytocin, ACh, or PGF2α.-induced smooth muscle contraction. Moreover, they are non-competitive inhibition .TPS inhibits the high potassium ( KCl 56. 3 mM)-induced cellular depolarizortion and the oxytocin- induced rhythmic cont ractions. Furthermore, TPS inhibits oxytocin-induced uterus smooth muscle cont raction in Ca2+-free solution. The results suggest that TPS might inhibit Volt age operated calcium channels(VOCs) and /or Receptor operated calcium channels (ROCs) on the uterus smooth muscle. Taken together, these results suggested th at the action of secure childbirth of TPS was due to the inhibtion of uterus s mooth muscle. The possible physiological mechanisms of TPS on the tocolygic ef fect was the non-competitive inhibition with oxytocin, ACh, and PGF2α and the inhibition of both calcium influx and calcium release from calcium store.