- 學位論文
攝取有機飲食與體內抗氧化酵素活性及銅、硒濃度之相關性研究
The Relationship among Superoxide Dismutase, Glutathione Peroxidase and Trace Metals for Organic Diet Women
摘要
近年來，有機飲食隨著健康意識抬頭之風潮而盛行，所謂「有機飲食」不僅限於蔬果類，而是所有以有機栽培所種植之農作物或不用人工化學藥物生產之肉類、奶製品等為食物之飲食方式均可稱之。另一方面，飲食又為人體內活性氧物質之最大來源，因此，一般民眾相信自然、無農藥、化學肥料人工藥劑污染之有機飲食能夠阻斷活性氧物質之侵襲，達到預防疾病、對身體健康有益之效。因此，本研究目的為：一、瞭解一般民眾接受有機飲食之原因，二、檢測人體血中抗氧化酵素活性與微量元素濃度之相關性，三、分析有機飲食對人體內抗氧化酵素活性與微量元素濃度之影響性，四、分析其他影響人體內抗氧化酵素與微量元素之因子，五、評估有機飲食對人體健康之影響 本研究選取共29位年齡在19-67歲之女性，以問卷調查基本人口學、生活型態及飲食型態資料，並採集其血樣5-10mL，以供檢測麩胱甘肽過氧化酶（Glutathione Peroxidase, GSH-Px）、超氧化物歧化酶（Superoxide Dismutase, SOD）活性及銅、硒濃度。 結果發現，有35%者認為一般蔬果農藥殘留太多，26%者則為了健康而選擇有機飲食；有機飲食組之GSH-Px活性（4085.84±1832.83 U/L）顯著地較非有機飲食組（5600.60±1447.87 U/L）低，但經過複回歸分析調整其他因子後，有機飲食之攝取與GSH-Px活性之相關性消失。而有機飲食組之SOD活性（196.41±48.91 U/mL）顯著地較非有機飲食組（116.43±52.08 U/mL）高（p<0.001），在經過複回歸分析調整其他因子後，有機飲食之攝取與SOD之相關性，即攝取有機飲食可顯著地提升體內SOD之活性。兩組間之銅、鋅濃度則無顯著差異。且在本研究中也發現，有機飲食之攝取，或許可以修補生活或飲食型態不正常所造成之抗氧化系統不平衡。另一方面，年齡、牡蠣之攝取頻率與吸菸頻率則是影響GSH-Px活性之主要因素；而肉類攝取頻率則與SOD活性有顯著的負相關（p<0.05）。每日平均攝取蔬果量也會影響抗氧化酵素活性，但是影響的程度不及有機飲食之攝取與否。 整體而言，有機飲食之攝取對人體內抗氧化酵素系統是有助益的，然而良好之生活型態及適當之飲食，亦可對人體內抗氧化酵素活性有提升之作用。
並列摘要
“Organic diet” is more popular. The way that the food we eat grow without chemicals and artificial and with organic farming called “Organic diet”. In the other hand, dietary is the dominant source which reactive oxygen species in human body come from. Accordingly, it is believed that “Organic diet” would protect cells from the attack of reactive oxygen species and could prevent diseases. The purpose of this study is to realize that why people choose to have “Organic diet”, to understand the correlation of the activities of antioxidant enzymes (GSH-Px, SOD) and the concentrations of trace metals (Cu, Se) in human blood, to analyze the level of organic diet and other factors affect antioxidant enzymes and trace metals and to assess the effect of organic diet to health. 29 women (aged 19-67) is selected. The present study collected their demographic data, lifestyle and dietary factors and also collected whole blood 5-10mL to detect the activities of GSH-Px, SOD and the concentrations of Cu, Se. The dominant reasons of having organic dietary is that there are 35% of subjects is for decrease the exposure of chemicals and 26% of subjects is for health. The women who have organic dietary have significantly lower GSH-Px activities (4085.84±1832.83 U/L) than the women who don’t have organic dietary (5600.60±1447.87 U/L), But after multiple regression analysis, the correlation of organic dietary and GSH-Px activities is not real. Furthermore, The women who have organic dietary have significantly higher SOD activities (196.41±48.91 U/L) than the women who don’t have organic dietary (116.43±52.08 U/L) (p<0.001), and after multiple regression analysis, the correlation of organic dietary and SOD is still present. It means that organic dietary could significantly increase SOD activity. In present study, eating organic dietary may adjust the imbalance of antioxidant system in human body which result from abnormal lifestyle or eating habits. Besides, age, intake frequency of oyster and smoking frequency are also important factors affect GSH-Px activity. Eating vegetables and fruits may also affect the activities of GSH-Px and SOD, but the effect is less than eating organic diet. In conclusion, eating organic dietary is good for antioxidant enzymes in human body, and adequate lifestyle and dietary also increase the activities of antioxidant enzymes.