- 學位論文
止痛生醫材料在疼痛控制的評估
Evaluation of analgesic biomaterials in pain control
摘要
疼痛控制對於要接受手術的患者非常重要。潛在的慢性和持續性的手術後疼痛會對患者的生活品質和經濟能力產生負面影響。通常使用於疼痛治療的止痛劑包括鴉片類藥物和非類固醇抗發炎性藥物等。然而，這些止痛藥有副作用、止痛效果短、和成癮風險的缺點。考慮到每年進行的手術數量，開發更有效的止痛藥物和止痛生物材料是非常值得的。我們利用一個神經性疼痛大鼠行為模型來評估止痛生物材料的有效性。首先我們暴露大鼠一側的坐骨神經，並穿過神經綁上尼龍縫線結。這一個線結壓迫了三分之一的坐骨神經截面以形成一個局部坐骨神經傷害。然後我們將載有0%、10%和20% 利多卡因的三種聚己内酯薄膜包覆在三組大鼠的神經上作為止痛。手術完成後，將利用von Frey test (機械異常性疼痛)和Hargreaves’ method (熱痛覺過敏)做疼痛反應測試。通過測量大鼠的觸碰刺激後爪退縮門檻和熱刺激後爪退縮延遲，我們可以判斷到這是否是評估大鼠疼痛的有效模型，以及生物材料薄膜是否有效的減輕了大鼠的疼痛。結果載有20％的利多卡因生物材料薄膜組的後爪退縮門檻和後爪退縮延遲都有顯著的增加，表示載有利多卡因的生物材料薄膜具有鎮痛作用。
並列摘要
Pain control is of great significance to patients receiving a surgical operation. The potential long term and persistent postoperative pain can negatively affect a patient’s quality of life and economic productivity. Analgesics commonly used for pain control include opioids, non-steroidal anti-inflammatory drugs, and others. These analgesics however have drawbacks such as side effects, short duration of therapeutic effect, and risks of addiction. Considering the amount of operations performed each year, it is of great importance to develop more effective analgesic drugs and biomaterials less burdened by such drawbacks. We utilize a rat model of neuropathic pain to evaluate the effectiveness of analgesic biomaterials. First we created a partial sciatic nerve injury by exposing the sciatic nerve, inserted a suture through the nerve to form a ligation that constricts a third of the nerve’s thickness. We then attach three different polycaprolactone films with 0%, 10%, and 20% lidocaine in three separate groups upon the sciatic nerve for analgesia. After the operation is performed, von Frey hairs (mechanical allodynia) and infrared stimulation (heat hyperalgesia) are tested on the rats’ paws over several days. By measuring the paw withdrawal threshold to touch and paw withdrawal latency to heat from the rats, we may see if this is an effective model for evaluating biomaterial analgesic effect within a rat, and whether the lidocaine carrying biomaterial film effectively reduces pain within the rats. The result was the group with 20% lidocaine biomaterial film has shown significantly increased paw withdrawal threshold and increased paw withdrawal latency, indicating that the lidocaine carrying biomaterial film has an analgesic effect.