摘要
本研究乃是將豆科植物，野百合屬太陽麻 (Crotalaria juncea L.)進行成分分離純化並進行生物活性探討。台灣產太陽麻2004年6月採於屏東縣，取莖部350 g，室溫下以甲醇冷浸約一星期後，將其抽出液經減壓濃縮後之抽出物50 g。以矽膠柱層層析法分離，共分離得到十四個化合物，其中包含十二個已知化合物分別為β-Sitosterol (1)， β-Sitosterol-3-O-β-D-glucoside (2)，Betulinic acid (3)，β-Amyrin (4)， 22-Episoyasapogenol B (5)，Genistein (6)，Daidzein (7)，7,4?S-Dihydroxyflavone (8)，3?S,4?S-Dihydroxy-7-methoxyflavone (9)，3?S,4?S-Dihydroxy-5,7-methoxyflavone (10)，Crotafuran A (13)及 Crotafuran B (14)。另有二個新化合物為 Crotafuran F (11) 及Crotafuran G (12)。 將化合物4進行抗發炎 (anti-inflammatory effect) 生物活性之篩選，結果顯示以PMA (3 nM)所誘發老鼠嗜中性白血球形成超氧陰離子 (superoxide anion generation)的作用中，呈現抑制的活性且抑制能力與濃度相關，其IC50值為15.9 ?b 2.3 µM。
關鍵字
太陽麻並列摘要
With the scope of our search for biologically active compounds from plants, twelve known compounds, β-Sitosterol (1), β-Sitosterol-3-O-β-D- glucoside (2), Betulinic acid (3), β-Amyrin (4), 22-Episoyasapogenol B (5), Genistein (6), Daidzein (7), 7,4?S-Dihydroxyflavone (8), 3?S,4?S- Dihydroxy-7-methoxyflavone (9), 3?S,4?S-Dihydroxy-5,7-methoxyflavone (10), Crotafuran A (13), and Crotafuran B (14), and two new compounds, Crotafuran F (11) and Crotafuran G (12), were isolated from the stem of Crotalaria juncea L.. Their structures were determined by spectroscopic methods and comparison with those data reported in literatures. Compound 4 showed significant inhibitory effects on the superoxide anion generation in rat neutrophils stimulated with PMA (3 nM) in a concentration-dependent manner with an IC50 value of 15.9 ?b 2.3 µM.