- 學位論文
牡丹皮、天門冬精華液(Ps. Ac. lotion)在減輕女性臉部皮膚暗沉之研究
Ps. Ac. lotion containing the extract of Paeonia suffruticosa and Asparagus cochinchinensis attenuated skin pigmentation in women: A pilot study
摘要
黑色素過量產生是導致皮膚色素沉著(Skin Hyperpigmentation Disorders，SHD)的主因，如肝斑，雀斑及發炎後色素沉著。近幾十年來輔助和替代醫學(Complementary and Alternative Medicine，CAM)變得越來越常被使用。傳統中醫(Traditional Chinese Medicine，TCM)屬於CAM的一種療法，認為當人體長期處於體液缺乏(陰虛)，血瘀和血熱會造成SHD。臨床中醫通常使用滋陰中藥，如天門冬(Asparagus cochinchinensis，Ac)來滋養人體體液及皮膚以改善皮膚新陳代謝並使皮膚達到美白。TCM還利用活血化瘀中藥如牡丹皮(Paeonia suffruticosa，Ps)來促進血液循環並消除血瘀來治療SHD。先前研究已證實Ps和Ac萃取物有效地抑制黑色素細胞的酪氨酸酶活性，但尚未有相關實證醫學研究來探討其皮膚美白功效。因此本研究目的在探討含有Ps及Ac萃取物的Ps. Ac. 精華液對女性SHD之改善效果。本研究是一項隨機單盲臨床試驗，共有60位年齡為25至60歲健康女性受試者，其Fitzpatrick皮膚類型為III及IV型，並依循中醫體質量表及舌診評估其體質後，再將其隨機分為3組，0.5% Ps. Ac. 精華液，2% Ps. Ac. 精華液及未含Ps及Ac萃取物精華液之安慰劑，每組各20人。受試者每日2次臉部清洗後塗抹樣品於皮膚暗沉部位。之後在試驗前(第0週)及試驗後(第8週)經數位膚質檢測儀(VISIA)評估臉部黑色素程度。研究實驗結果顯示，不同組別的受試者確實有皮膚色素沉著狀況，但其在中醫體質量表下的個人體質無統計上顯著差異(p>0.05)。在使用後的第8週，0.5%及2% Ps. Ac. 精華液確實改善受試者全臉的皮膚表淺斑點(p<0.05)。綜上所述，Ps. Ac. 精華液含有牡丹皮及天門冬萃取物對皮膚色素沉著的受試者具有良好美白效用，在未來可做為皮膚嫩白的保養品原料應用在化粧品或醫藥品產業上。
並列摘要
Melanin overproduction causes several skin hyperpigmentation disorders (SHD), such as melasma, freckle and post-inflammatory pigmentation. Complementary and Alternative Medicine (CAM) has become increasingly popular in the past decades. Traditional Chinese Medicine (TCM), one form of CAM, has indicated that long term body fluid deficiency (津液缺乏), blood stasis (血瘀) and blood heat (血熱) can cause SHD. Clinical TCM usually uses the enrich yin (滋陰) herb, such as Asparagus cochinchinensis (Ac), to nourish the human body fluid and moisturize the skin for improving skin metabolism, resulting in skin whitening. In addition, TCM also uses the angiogenesis promoting herb, such as Paeonia suffruticosa (Ps), to cure SHD through promoting blood circulation to dispel blood stasis (活血化瘀). Previous studies have revealed that the extract of Ps and Ac can effectively inhibit tyrosinase activity in melanocytes, but the evidence base for its skin whitening effect has not been investigated yet. In the present study, Ps. Ac. lotion containing the extract of Ps and Ac was used to attenuate skin pigmentation in female subjects through a pilot study. This is a randomized, placebo-controlled, single-blind clinical trial. Sixty female subjects aged between 25 and 60 were enrolled, and their Fitzpatrick skin types were type III to IV Asian skin with hyperpigmentation conditions. There are three randomized groups in this pilot study, including 0.5% and 2% Ps. Ac. lotion and placebo, with 20 subjects in each group. The body constitution of subjects in each group was evaluated by Constitution in Chinese Medicine Questionnaire and tongue diagnosis. All subjects in each group were administered various test samples in facial pigmentation areas twice a day after cleaning. Before (0 week) and after (8 week) treatment, all subjects were non-invasively evaluated by VISIAR photography. The results indicated that there were no significantly differences in constitution and facial skin pigmentation in each group (p>0.05). There was a significant decrease in superficial spots on the entire face after using 0.5% and 2% Ps. Ac. lotion (p<0.05). In summary, Ps. Ac. lotion can effectively improve the skin pigmentation of subjects and displays skin whitening effect, and we suggest that Ps. Ac. lotion may be used as a skin whitening product in the cosmetic or pharmaceutical industry in the future.