- 學位論文
利用毛細管電泳法進行尿液中同化性代謝雄性荷爾蒙之檢測分析
Determination of anabolic androgenic steroids in urine by capillary electrophoresis
摘要
本論文建立一種分析androstenedione, testosterone, epitestosterone, boldenone及clostebol之線上堆積毛細管電泳法。本方法利用液相萃取法 (萃取溶媒為正己烷) 進行尿液樣品前處理後，於熔矽毛細管 (全長61 cm，有效分析長度 50 cm，內徑 50 μm) 通入分離用之磷酸鹽緩衝溶液 (30 mM，pH 5.0) 內含 100 mM sodium dodecyl sulfate 和40 % methanol以10 psi壓力取樣99.9秒，於 -20 kV電壓進行分離，以254 nm波長偵測之。方法經由確效後，其定量範圍為50-1000 ng/mL，相關係數皆大於0.990，展現良好的線性關係；評估同日內及異日間分析的精確度及準確性，其RSD和RE皆小於6.6%，且偵測極限可達30 ng/mL (S/N = 5，以10 psi壓力取樣99.9秒)，本研究方法擬實際運用於運動禁藥之檢測。
並列摘要
This study describes approaches for stacking and analyzing of androstenedione, testosterone, epitestosterone, boldenone and clostebol in urine by sweeping capillary electrophoresis. After liquid-liquid extraction with n-hexane, the extracted samples were loaded by hydrodynamic injection (10 psi, 99.9 s). The stacking and separation were performed at -20 kV and detected at 254 nm using phosphate buffer (30 mM, pH 5.0) containing 100 mM sodium dodecyl sulfate and 40% methanol. During method validation, calibration curves were linear (r ≥ 0.990) over a range 50 - 1000 ng/mL. For the evaluation of precision and accuracy, the RSD and RE were all less than 6.6% in intra- and inter-day analysis. The limits of detection were 30 ng/mL (S/N = 5, sampling 99.9 s at 10 psi). This study could be applied for doping monitoring.