- 學位論文
吸菸組與對照者之尼古丁乙醯膽鹼受體基因多型性對肥胖指標及生化指標的影響
The effects of nicotinic acetylcholine receptors gene polymorphism on obesity index and biochemistry between smokers and control.
摘要
抽菸已是公共衛生上一個重要的議題，研究發現香菸中主要成分-尼古丁，在進入人體後和乙醯膽鹼接受體結合，進而刺激多巴胺的釋放。神經型的乙醯膽鹼接受體由5個次單位所組成，總共有12種次單位，包含9種類型的α次單位(α2~α10)以及3種類型的β次單位(β2~β4)。文獻指出，尼古丁乙醯膽鹼接受體的變異或基因多型性可能影響生理功能。舉例而言研究發現CHRNA2的基因多型性可能是肥胖和過重的危險因子。 本研究目的為探討抽菸和對照組之間，尼古丁乙醯膽鹼接受體的基因多型性和生理指標的相關性。 這是一個案例對照研究(case-control study)，本研究納入2010年1月至12月期間116位吸菸者以及116位對照個案(健康成年人)，收集的資料包括人口學變項、身體肥胖指標(包括身高、體重、BMI、腰圍和臀圍)、血壓，並收集這些個案的空腹血液進行血液生化值以及尼古丁濃度的分析，血液同時進行DNA萃取及基因多型性分析。 基因多型性的分析是採用RT-PCR方法，檢測的乙醯膽鹼接受體基因型包括CHRNA3 rs7177514、CHRNB4 rs11636605 、CHRNA5 rs17486278、CHRNA7 rs6494223、和CHRNG rs2099489。 本研究結果顯示CHRNB4 rs11636605 minor allele(G)與尼古丁成癮有顯著相關；同時CHRNB4 rs11636605 minor allele(G)對體重和BMI的增加也有顯著相關，兩者都達到統計上顯著性。
並列摘要
Cigarette smoke is an important issue in public health. Some studies find that the majar composition of the cigarette, nicotine , release into the blood flow and integrate with nicotinic acetycholine receptors (nAChRs), and mediate to the release of Dopamine.Nicotinic acetycholine receptors (nAChRs) include muscle and neuronal receptors. Neuronal receptors constitute by 5 subunits, includes α2~α10 and β2~β4. Prior researches suggested that the variations of nAChRs gene polymorphisms may influence some kind of physical functions. For instance, Korea study found that CHRNA2 gene polymorphism may be related to overweight/obesity. The purpose of this study is to investigate the association, among nAChRs gene polymorphisms, cigarette smoking, and biochemical biomarkers. Therefore, this case-control study recruited a total of 232 participants, 116 Smokers and 116 reference (health adults), in the year 2010 in a Community hospital. Collected data include demographics, body obesity index (including BH, BMI, the waist, the hip), and blood pressure. Fasting blood sample were also collected and plasma biochemical and nicotine metabolism were analyzed.DNA was also extracted for Genotyping. Genotyping was performed using TaqMan R SNP Genotyping Assays. Gene polymorphism analyses included CHRNA3 (rs7177514), CHRNA5 (rs17486278), CHRNA7 (rs6494223, rs2337980), CHRNB4 (rs11636605) and CHRNG (rs713041). Our results showed that CHRNB4 rs11636605 was associated with increase weight and BMI. CHRNB4 rs11636605 might be a risk factor for weight and BMI especially in the Smokers.