- 學位論文
硫酸化軟骨素-丙烯酸甲酯與聚乙二醇-二丙烯酸酯共聚物水膠的製備及分析
Preparation and Characterization of Methacrylate Chondroitin Sulfate – co –Polyethylene Glycol Diacrylate Hydrogel
摘要
硫酸化軟骨素(Chondroitin sulfate，CS)為一已知天然多醣體，具有良好的生物相容性（Biocompatibility）和生物可降解性（Biodegradability）。本實驗使用硫酸化軟骨素來製備水膠以作為遞送蛋白質藥物的載體。實驗首先準備三種不同分子量的聚乙二醇二丙烯酸酯（Poly ethylene glycol-diacrylate）和甲基丙烯酸酯基（Methacrylate，MA）接枝率0.28的硫酸化軟骨素-丙烯酸甲酯（Chondroitin sulfate-methacrylatae），並使用這三種不同分子量的PEG-DA且三組CS-MA / PEG-DA比例來製備水膠，共有九種水膠。以此九種水膠進行性質分析，如重量損失測量、膨潤程度測量及壓縮模數測量，並使用掃描式電子顯微鏡（Scanning Electron Microscope，SEM）觀察水膠的表面形態，最後利用這九種水膠包覆牛血清白蛋白（Bovine serum albumin，BSA）來模擬蛋白質藥物的釋放。實驗結果顯示水膠有20~30 %的重量損失。水膠膨潤程度有10~20倍，固定CS-MA / PEG-DA時使用低分子量的PEG1K-DA其膨潤效果要比使用PEG2K-DA 和PEG4K-DA更佳；而固定PEG-DA分子量時則CS-MA / PEG-DA比越高則膨潤效果越佳。壓縮模數G值的高低 與CS-MA / PEG-DA比成正比而與PEG-DA分子量成反比。水膠釋放FITC速率和其膨潤成正比，膨潤效果較佳的低分子量PEG-DA所製成的水膠其釋放也是最為迅速，而分子量較高的PEG-DA製成的水膠相對釋放較緩慢。另外改變不同的CS-MA / PEG-DA比例對於釋放較不顯著。
並列摘要
The goal of this work was to utilize the colon-specifically degradable polymer，chondroitin sulfate (CS)，prepare hydrogel for a protein delivery Polyethylene glycol diacrylate (PEG-DA) with the different molecular masses of PEG (1K, 2K, and 4K g/mol) and methacrylated chondroitin sulfate (CS-MA) with 28 mol% of methacrylated substitution onto chondroitin sulfate were first prepared. Three hydrogels with different ratios between PEG-DA and CS-MA were synthesized and their effects on weight loss, swelling, compression modulus, and controlled release of bovine serum albumin were carried out. We found that about 20 - 30 weight loss were obtained and slightly decreased with the increase in molecular masses of PEG after water extraction of these hydrogels. The swelling percentage all went up with the increase in the contents of CS-MA in hydrogels using three different molecular masses of PEG and especially pronounced in the PEG1K hydrogels. The degree of swelling were within 10-20 times. In an example of PEG-DA with the PEG2K, the compression modulus and effective crosslinking density increased with the ratio increase of CS-MA to PEG-DA. In in vitro drug released studies using FITC-BSA as a model drug. The results showed that the release rates of CS-MA-co-PEGDA hydrogel went hand-in-hand with the swelling data. The high swelling percentages in PEG-DA with lower molecular weight of PEG led to the higher release rate of FITC-BSA. However, the effect of the ratio between CS-MA and PEG-DA on the release rate of FITC-BSA was unpronounced.