- 學位論文
利用毛細管液相層析法分析市售化粧品中脂溶性抗氧化劑
Determination of Hydrophobic Antioxidants in Commercial Cosmetic Products by Capillary Liquid Chromatography
摘要
本研究利用毛細管高效能液相層析法結合UV偵測器，對於市售化粧品和飲食補充品中之coenzyme Q10、idebenone、retinol、α-tocopherol及phylloquinone建立簡單且高感度的定量分析法。因分析物之特性，以n-hexane作為萃取之溶劑。分析的條件為使用C18 （150 x 0.5 mm；粒徑5 μm）的毛細管層析管柱，分析coenzyme Q10的移動相組成為methanol/isopropanol（50/50, v / v），流速10 μL/min，採等梯度沖提；分析idebenone、retinol、α-tocopherol及phylloquinone的移動相組成為methanol/water（70/30, v / v），流速10 μL/min，採梯度沖提，於一分鐘之內將移動相組成增至100% methanol。所得之coenzyme Q10、idebenone、retinol、α-tocopherol及phylloquinone線性範圍分別為0.25-25、0.01-0.8、0.07-5.6、0.7-56和0.25-20μg/mL，r2分別為0.999、0.996、0.999、0.999和0.998，偵測極限（s/n=5）分別為0.027、0.001、0.008、0.1206和0.0546 μg/mL。
並列摘要
Simple and sensitive capillary liquid chromatography methods couple with UV detector are described for the determination of coenzyme Q10, idebenone, retinol, α-tocopherol and phylloquinone in commercial cosmetic and nutrition supplement products. The separation methods are performed on capillary liquid chromatography with C18 column (150 x 0.5 mm; 5 μm particle size). Coenzyme Q10 was analyzed by isocratic elution of methanol/isopropanol (50/50, v/v). Idebenone, retinol, α-tocopherol and phylloquinone were simultaneously separated by gradient elution, the gradient elution was used as following: 70% methanol for fist minute then 100% methanol for 1-17 minute. The linear ranges of coenzyme Q10, idebenone, retinol, α-tocopherol and phylloquinone are 0.25-25, 0.01-0.8, 0.07-5.6, 0.7-56 and 0.25-20μg/mL, respectively. The correlation coefficients are 0.999, 0.996, 0.999, 0.999 and 0.998, respectively. The detection limits (s/n=5) are 0.027, 0.001, 0.008, 0.1206 and 0.0546 μg/mL, respectively.