Active anti-hyperuricemia ingredients in Chrysanthemum indicum Linn. inhibits xanthine oxidase and reduces serum uric acid in rats.

菊花之萃取成份可抑制黃嘌呤氧化酶以及降低大鼠血液中的尿酸

李安哲(An-Che Li)

Advisor ： 黎耀基

國立清華大學/生命科學院/生物科技研究所/碩士(2011年)

https://doi.org/10.6843/NTHU.2011.00267

Abstracts

痛風是一種代謝性疾病，其特點是反覆發作的急性關節發炎症，這是由於血液中尿酸結晶的升高，並沉積在關節，肌腱和周圍組織。黃嘌呤氧化酶(xanthine oxidase)催化次黃嘌呤(hypoxanthine)氧化成為黃嘌呤(xanthine)最後成為尿酸，在高尿酸血症裡扮演一個重要的角色。野菊花(Chrysanthemum indicum Linn)是一種流行的傳統草藥，目前在台灣和中國被廣泛的使用在中藥上面，這種草藥目前被用來治療各種免疫相關疾病、高血壓和一些傳染性疾病。在這項研究中，我們研究菊花提取物和別嘌呤醇(allopurinol)在黃嘌呤氧化酶抑制實驗和高尿酸大鼠中，對於尿酸下降的影響。結果顯示，白菊花乙酸乙酯提取物呈現最強的黃嘌呤氧化酶抑制活性(IC50=30.3 μg/mL)，而白菊花正己烷和水的提取物沒有明顯的黃嘌呤氧化酶抑制活性。在大鼠的研究中，我們發現給予劑量300 mg/kg 的白菊花乙酸乙酯提取物，可以減少動物血清尿酸濃度至2.28 ± 0.13 mg/dL，高尿酸血症對照組為4.40 ± 0.08 mg/dL。

Keywords

高尿酸血症 ； 黃嘌呤氧化酶 ； 野菊花 ； 痛風

Parallel abstracts

Gout is a metabolic diseases usually characterized by recurrent attacks of acute inflammatory arthritis. It is caused by elevated levels of uric acid in the blood which crystallize and are deposited in joints, tendons, and surrounding tissues. Xanthine oxidase catalyses the oxidation of hypoxanthine to xanthine and then to uric acid, which plays a crucial role in hyperuricemia. Chrysanthemum indicum Linn is one of the popular traditional herbs extensively used in Taiwan and Chinese medicine. The herb have been used to treat various immune-related disorders, hypertension symptoms and several infectious diseases. In this study, we investigated effects of C. indicum extracts on xanthine oxidase activity, a key enzyme in uric acid synthesis, uric acid levels in hyperuricemic rats. The results demonstrated that the ethyl acetate extract of C. indicum presented the strongest xanthine oxidase inhibition activity (IC50=30.3 μg/mL). However, no significant xanthine oxidase inhibition activities were found in the hexane and water extracts. We also found that administration of a single dose of 300 mg/kg ethyl acetate extract of C. indicum to such animals reduced the serum uric acid concentration to 2.28 ± 0.13 mg/dL, compared with a concentration of 4.40 ± 0.08 mg/dL in the hyperuricemic control group.

Parallel keywords

Hyperuricemia ； Xanthine oxidase ； Chrysanthemum indicum Linn. ； Gout

References

46.黃彩鳳. (2005) 洛神花萃取物降低鼠體血中尿酸之作用, 中山醫學大學生化暨生物科技研究所碩士論文.

3.Sloan, R. W. (1982) Hyperuricemia and gout, J Fam Pract 14, 923-926, 930-921, 934.

4.Martinon, F., Petrilli, V., Mayor, A., Tardivel, A., and Tschopp, J. (2006) Gout-associated uric acid crystals activate the NALP3 inflammasome, Nature 440, 237-241.

5.Mazzali, M., Hughes, J., Kim, Y. G., Jefferson, J. A., Kang, D. H., Gordon, K. L., Lan, H. Y., Kivlighn, S., and Johnson, R. J. (2001) Elevated uric acid increases blood pressure in the rat by a novel crystal-independent mechanism, Hypertension 38, 1101-1106.

6.Khan, S. R. (2004) Crystal-induced inflammation of the kidneys: results from human studies, animal models, and tissue-culture studies, Clin Exp Nephrol 8, 75-88.