- 學位論文
以微流體式血型辨識晶片進行紅血球凝集量測
Determination of degree of RBC agglutination using microfluidic blood typing chip
摘要
醫療手術中血液分型檢測是非常重要的一部份，確保接受者血液與捐贈者血液相容，輸血中的血型錯誤可能導致血管內產生溶血，腎衰竭以及休克等情形。本論文提出了一種微流體血型辨識系統，使用少量血液樣品來確定紅血球的凝集程度。該微流體血型辨識晶片包含五個注入槽及檢測槽，並將指叉電極(IDE)製作於檢測槽內用來量測血液的凝集程度。此外，提出了兩種測量方法：阻抗量測和電性量測；阻抗量測中的電荷轉移電阻和電性量測中的功率參數用來分析凝集程度，從兩種測量方法的結果顯示，其參數與紅血球凝集程度呈現線性關係。然而受到許多因素的影響如晶片的狀況，使電性分析比阻抗量測方法來的可靠。 提出此方法能夠明確的定義各個凝集程度的值，並且區分從非凝(0級)到強烈凝集(4級以上)的五個等級，符合臨床要求，以防止輸血中的任何風險。
並列摘要
Blood typing assay is a critical test to ensure the serological compatibility of a donor and an intended recipient prior to a blood transfusion. Mistyping of blood group in transfusion might result in intravascular hemolysis, renal failure and shock. This thesis presents a microfluidic blood typing system using a small quantity of blood sample to determine the degree of RBC agglutination. This microfludic blood typing chip comprises five injection inlets and a detection chamber. An interdigitated electrode (IDE) array, acting as a sensor for the measurement of blood agglutination, exists inside the detection chamber. Moreover, two measuring methods were proposed: impedimetric measurement and electroanalytical measurement. The charge transfer resistance in the impedimetric measurement and the power parameter in the electroanalytical measurement were used for the analysis of agglutination level. From the experimental results, both measuring methods provide quantitative results and the parameters are linearly and monotonically related with the degree of RBC agglutination. However, the electroanalytical measurement is more reliable than the impedimetric technique because the impedimetric measurement may suffer from many influence factors, such as chip conditions. Five levels from non-agglutination (level 0) to strong agglutination (level 4+) can be discriminated, conforming to the clinical requirement to prevent any risks in transfusion. This proposed approach provides unambiguous quantitative identification of agglutination level for blood typing.