- 學位論文
人參皂苷Rb1對於神經幹細胞增生與分化的影響
Effects of Ginsenoside Rb1 on Proliferation and Differentiation in Neural Stem Cells
摘要
人參為亞洲傳統的中藥材，且使用已經有幾千年了，而最具有藥理活性的物質為人參皂苷 (ginsenosides)，人參皂苷已被證實具有多種功效，例如改善心血管疾病、調節中樞神經系統、增進免疫功能、抗癌、幫助學習及記憶等。先前研究結果顯示，人參皂苷Rb1 (ginsenoside Rb1) 會促使小鼠囊胚期胚胎 (mouse blastocytes) 走向粒線體依賴 (mitochondria-dependent) 的凋亡 (apoptosis) 機制，所以GRb1會影響老鼠胚胎的發育。因此本篇研究想探討GRb1對於體外培養神經幹細胞其增生和分化影響為何，神經幹細胞 (neural stem cell) 具有自我更新、分化的潛能，在神經系統疾病或神經的損傷修復，神經幹細胞扮演重要的角色。本研究利用會促使小鼠囊胚凋亡的GRb1的濃度，結果顯示100 μg/ml GRb1能抑制神經球 (neurospheres) 的增長，也發現神經幹細胞的增生數目減少。相反的，類似於cAMP的dibutyryl cAMP (dbcAMP) 與100 μg/ml GRb1共同培養，會提升神經幹細胞神經突的生長 (neurite outgrowth)。因此本研究認為GRb1對於神經幹細胞的分化與增生的效用是相反的。
並列摘要
Ginseng has been widely used as traditional medicine for several thousand years. Many studies were established to investigate the role of Ginsenoside Rb1, one of the major active compounds found in ginseng, in various types of cells. Ginsenoside Rb1 was proved has a variety of biomedical efficacies such as reversing the cardiovascular diseases, protecting immune systems, enhancing learning and memory, modulating central nervous systems (CNS), and even preventing cancer. Conversely, another study showed that Ginsenoside Rb1 induced mouse blastocyst apoptosis through mitochondria-dependent apoptotic pathway. They assumed Ginsenoside Rb1 may affect the mouse embryonic development. Thus, we want to explore the effect of ginsenoside Rb1 on proliferation and differentiation of Neural stem cells (NSCs) in vitro. NSCs, self-renewing and differentiation potential cells, play an important role in rebuilding the nervous system. In the present study, NSCs were treated with Ginsenoside Rb1 at the concentration that significantly induced apoptosis in mouse blastocyst, 100 μg/ml. Results showed that 100 μg/ml Ginsenoside Rb1 reduced the neurospheres size. We also found that 100 μg/ml Ginsenoside Rb1 could inhibit the proliferation of NSCs. In contrast, dibutyryl cAMP (dbcAMP), cAMP analog, and 100 μg/ml Ginsenoside Rb1 co-incubation for 1 week enhanced the neurite outgrowth. Therefore, we suggest that Ginsenoside Rb1 possess the opposite regulation in both NSCs proliferation and differentiation.