- 學位論文
抗菌奈米銀絲素蛋白複合纖維之製備與其表面性能之研究
Study on the preparation and surface properties of nano silver silk fibroin composite fiber
摘要
本研究使用生物可降解並具有良好生物相容性的天然絲素蛋白(Silk fibroin)作為原料，以靜電紡絲技術將其製成奈米纖維，並透過其內部的酪胺酸(Tyrosine)殘基以及光輔助法，以LED燈在室溫下，僅使用硝酸銀溶液，可使奈米銀在纖維內生長，而製成抗菌奈米銀絲素蛋白複合纖維。本實驗透過改變絲素蛋白的結晶度、LED光源波長，以及照光實驗的條件，來改變奈米銀之生長條件，並以SEM、UV-vis與XRD，分析複合纖維表面特性及結晶狀況，最後透過圓盤擴散法，測試其抗菌能力。 實驗結果證實成功製備出奈米銀絲素蛋白複合纖維，經由分析纖維表面之形貌，發現隨著光源波長降低，以及照光時長增加，奈米銀數量及大小皆有增加的趨勢，形狀亦逐漸偏離球形，可出現立方體或三角平板的奈米銀顆粒;此外，酒精後處理，可增加絲素蛋白纖維之結晶度及水穩定性，並且其亦可使奈米銀更加均勻分布於其上，並使銀粒徑降低。再者，由複合纖維之側截面，可觀察到奈米銀顆粒非僅在纖維表面生長，而是平均分散在纖維內部。經由分析複合纖維在可見光波段之吸收光譜，發現到當奈米銀之粒徑增加、團聚及形狀變化時，除吸收位置紅移外，還出現第二吸收特徵峰;從XRD分析與抗菌試驗，確認銀奈米粒子之生成，且對大腸桿菌，具有抗菌效果。
並列摘要
In this study, natural silk fibroin, which is biodegradable and has good biocompatibility, was used as a raw material, and it was made into nanofibers by electrospinning technology. Through the tyrosine residues of silk fibroin and the light-assisted method, the silver nanoparticles are grown in the fibers from the silver nitrate solution at room temperature with a LED lamp to make antibacterial silver nanoparticles silk fibroin composite fibers. In this experiment, the growth conditions of nano-silver were changed by changing the crystallinity of silk fibroin, the wavelength of the LED light source, and the conditions of the light experiment. The surface characteristics and crystallization status of the composite fiber were analyzed by SEM, UV-vis, and XRD. Finally, the antibacterial ability was tested by disk diffusion method. The experimental results confirmed that nano-silver fibroin composite fibers were successfully prepared. By analyzing the surface morphology of the fibers, it was found that as the wavelength of the light source decreased and the duration of the light increased, the number and size of nano-silver tended to increase, and the shape gradually deviated from the spherical shape, which becoming cubes or triangular plates. In addition, alcohol post-treatment can increase the crystallinity and water stability of silk fibroin fibers, and it can also make nano-silver more uniformly distributed on it and reduce the silver particle size. Furthermore, from the cross-sectional area of the composite fiber, it can be observed that the nano-silver particles are not only grown on the surface of the fiber, but evenly dispersed inside the fiber. By analyzing the absorption spectrum of the composite fiber in the visible light band, it is found that when the particle size of nano silver increases, agglomerates and the shape changes, in addition to the red shift of the absorption position, a second absorption characteristic peak appears. From the XRD analysis and antibacterial test, it is confirmed that the formation of silver nanoparticles has an antibacterial effect on Escherichia coli.