台灣細本山葡萄抗凝血成分之分析與鑑定

Identification and characterization of anticoagulants from Vitis thunbergii var. taiwaniana

高君豪(Chun-Hao Kao)

指導教授 ： 吳立真

共同指導教授 ： 陳榮煇 ； 廖明淵

國立暨南國際大學/科技學院/應用化學系/碩士(2013年)

摘要

凝血和血栓形成的機制相似，抗凝血之物質能有效減緩血栓形成，預防心血管疾
病的發生。台灣產之細本山葡萄 (vitis thunbergii var. taiwaniana ) 為傳統食材及藥用植物。已被報導具有抗氧化、抗發炎、抗癌及抗凝血的效果。本研究為分析與鑑定台灣細本山葡萄內所含具抗凝血活性之化合物，將植株根部以 80%甲醇水溶液萃取後濃縮，加入異丙醇，進行鹽析步驟以在異丙醇層中獲得主要成分，異丙醇層再由管柱層析方法與高效能液相層析儀藉由活性導引分離方式，進行內源性凝血路徑( intrinsic-coagulation )及外源性凝血路（extrinsic-coagulation）之凝血時間測試確認活性部分後分離並純化，經由 NMR 鑑定，確認四個化合物為 (+)-catechin、amurensin G、hopeaphenol、(+)-viniferol D 其中 amurensin G、(+)-viniferol D 為首次於台灣細本山葡萄中發現。整體而言，台灣細本山葡萄可能有數種抗凝血成分與不同抗凝血機制，因此，台灣細本山葡萄值得進一步開發尋找新穎的抗凝血劑。

關鍵字

抗凝血 ； 細本山葡萄 ； 內源性凝血 ； 外源性凝血

並列摘要

Thrombosis and coagulation share similar mechanisms for clot formation. The use of anti-clotting substances may effectively reduce reduce thrombosis, and prevent the occurrence of cardiovascular disease. Vitis thunbergii var. taiwaniana, a conventional food and medical plant in Taiwan, has been reportedly to possess the bioactivities of anti-oxidation, anti-inflammation, anti-cancer, and anti-coagulation. The aim of this research was to analyze and identify the compounds with anti-coagulative activity in V. thunbergii var. taiwaniana. The roots were extracted with of 80% methanol and then concentrated under vacuum. The fraction was suspended by isopropanol, followed by the addition of salt to drive certain compounds to isopropanol layer. Separation in open column chromatography and HPLC were performed to isolate and purify anti-coagulants of isopropanol layer with the aid of bioassay-guided fractionation based on the time duration of intrinsic-coagulation and extrinsic-coagulation. NMR analysis revealed four potential anti-coagulants, (+)-catechin, amurensin G, hopeaphenol, (+)-viniferol D. Among which, amurensin G and (+)-viniferol D are the first time isolated from V. thunbergii var. taiwaniana. Collectively, the V. thunbergii var. taiwaniana may have several anti-coagulants with distinct anti-coagulaive mechanisms. Therefore, V. thunbergii var. taiwaniana may be a potential candidate for searching novel anticoagulants.

並列關鍵字

anticoagulation ； vitis thunbergii var. taiwaniana ； resveratrol ； intrinsic-coagulation ； extrinsic-coagulation

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