- 學位論文
於鄰苯二酚溶液中進行磺胺藥物的電化學反應與電分析應用
Electrochemical reaction and analytical application of sulfa drugs in the presence of catechol
摘要
目前偵測磺胺的方法主要有質譜、層析等，而在電化學分析上也有利用分子印或活化碳材進行檢測，但質譜與層析的成本高，在先前的電化學方法中也有著電流訊號不明顯、電極製作繁瑣的的問題。本研究先將羧酸官能化奈米碳管(carboxylic acid functionalized multi-walled carbon nanotube, 以MWCNT 表示) 修飾於網版印刷碳電極 (screen-printed carbon electrode, 以SPCE 表示) 上，接著將SPCE/MWCNT進行氧化還原處理，即成功製備氧化還原碳管修飾電極(SPCE/MWCNTRD)，並針對磺胺 (sulfanilamide, SAA)進行微量的定量分析。 利用循環伏安法(cyclic voltammetry, CV)偵測磺胺時，在 -0.2V ~ +0.8V的電位中觀測不到任何電流訊號，而在酸性環境下額外添加鄰苯二酚 (catechol, CA) 於分析溶液中後，CA會被電極表面氧化，促使醌胺聚合物(quinone-amine polymers , QAPs)生成，且能在+0.13V形成新的氧化還原對，且能利用QAPs的氧化電流來間接定量磺胺類藥物。利用差式脈衝伏安法(differential pulse voltammetry, DPV)進行磺胺的定量分析，線性範圍分別介於0.5-10 M 和10-50M，且此分析方法的最低極限檢測值為19.2 nM ，本研究利用簡單的修飾 方法，有效的降低偵測成本，並能大幅提前偵測電位。
並列摘要
The sulfonamide detection method mainly includes mass spectrometry and chromatography. In electrochemical detection, molecular imprinting technology and activated carbon materials are also used. However, mass spectrometry and chromatography are expensive, and the previous electrochemical methods also have a drawback that the current signal is not obvious and the electrode modification is complicated. In this study, carboxylic acid-functionalized multi-walled carbon nanotubes were first modified on a screen-printed carbon electrode, followed by electrochemical treatment, and then an activated carbon nanotubes modified electrode (SPCE/MWCNTRD) was successfully prepared. When using cyclic voltammetry to examine sulfanilamide in the presences of the potential range of -0.2V to +0.8V in an acidic environment, catechol will be oxidized on the surface of the electrode to promote the formation of quinone-amine polymers, and a new redox pair can be observed at +0.13V, which can be was use for quantification of the sulfonamide by differential pulse voltammetry (DPV). Two linear ranges of 0.5-10 μM and 10-50 μM were obtained. The lowest detection limit of this method was 19.2 nM. In this study, we use a simple modification method to effectively determine sulfanilamide.