We apply Puncher channel estimation to Orthogonal Frequency Division Multiplexing, (OFDM) communication system, using of two training symbols to estimate the channel response. In order to adapt to LTE-A system, we modified puncher channel estimation method into odd and even estimations. Q order time-varying polynomial curve are obtained in the case of OFDM symbol in a single duration, all of the sample points of the channel gain values can be obtained. We modified the puncher channel estimation method to adapt to LTE-A system. This method can provide better transmission efficiency in high mobility environments. The method works well in fast fading channel environments when the higher order polynomial is applied.