- 學位論文
新型生化感測器對寬頻帶光源之響應研究
Study on the Response of a Broaden Band Light Source for a New Type Biosensor Based on the Surface Plasmon Resonance Theory
摘要
本論文主要是提出有關新型生化感測器對寬頻帶光源之響應研究，此新型生化感測器的主要工作原理是利用表面電漿共振時，於此新型生化感測器之感測面會產生衰減全反射(attenuated total refection ATR)的現象，吾人可由光譜圖中共振谷發生時的共振波長去推得待測物質的相關特性，如：濃度、折射率與密度…等等。 本論文之新型生化感測器為一個或兩個直角稜鏡(Right-angle Prism)，其乃由折射率為1.51509之BK7材料所製成。吾人在稜鏡的斜邊鍍上兩層金屬膜，先鍍上2nm的鈦膜，再鍍上45.5nm的金膜。在本文中，採用250W寬頻帶的光源，並將此光源入射到直角稜鏡，我們可利用光譜儀得到待測物質於不同折射率時之光譜圖，當共振谷發生時可得到所對應的共振波長。如此，吾人可由光譜圖中共振谷發生時的共振波長去推得待測物質的相關特性。 本系統具有以下優點：可以即時量測、高解析度、高穩定度與架設簡單。吾人深信：此系統對未來生醫、生化或化學工業的研究發展可提供一個相當值得應用的工具。
並列摘要
In this thesis, a response for a broaden band light source on a new type biosensor based on the surface plasmon resonance theory is presented. From the spectrum diagram, the refractive index of the tested medium can be obtained by use of the corresponding wavelength in the resonant dip. The new type biosensor is one or two right-angle prisms made of BK-7 glass. The right-angle prisms are coated with 2nm Ti film and 45.5 nm Au film. In the paper, a broaden band light source with the wavelength range of 200nm~1100nm is used. As the light source is incident on the biosensor, we can achieve the spectrum diagram at the output of the biosensor by an optical spectrum analyzer. The refractive index of the tested medium can be obtained by use of the corresponding wavelength in the resonant dip. The method has some merits, e.g., a simple optical setup, high resolution, high sensitivity, rapid measurement, and high stability, etc. It could be valuable for biochemical, biotechnology and chemical industry in the future.