樟芝發酵脫脂黑芝麻粕水萃取物之ACE抑制活性研究

The ACE Inhibitory Activity of Water Extracts from Defatted Black Sesame Meals Fermented by Antrodia camphorata

郭信宏(Hsin-Hung Kuo)

指導教授 ： 張耀南

國立虎尾科技大學/文理學院/生物科技研究所/碩士(2014年)

摘要

此研究是以50 g/L的脫脂黑芝麻粕(DBSMs)加上10g/L的黑糖，利用被台灣人稱為〝紅寶石蘑菇〞的牛樟芝進行發酵，在28℃下深層培養14天，探討其水萃取物(Wets)對ACE 的活性抑制(ACEi)。發酵後DBSMs的水萃取物，其ACEi抑制率高於未發酵DBSMs的水萃取物。對水萃取物的比例(1:200, 1: 100, 1:50, 1:10, 1:5其定義為1 g冷凍乾燥的DBSMs分別添加200 mL ,100 mL ,50 mL ,10 mL ,5 mL水)進行ACEi的研究。水萃取物的ACEi隨著萃取率的增加而提高。另水萃取物的ACEi、水溶性蛋白及水溶性胜肽的含量隨著稀釋倍率的增加而減少。此外，當水溶性蛋白及胜肽的含量提高，其水萃取物的ACEi(%)會提升至接近飽和值(約88 %)。表示發酵時所產生的水溶性蛋白及胜肽有助於ACEi效果提升。水萃取物ACEi的乾重、水溶性蛋白及胜肽其IC50值，分別約為0.99, 0.19及0.14 mg/mL。而10 g/L的DBSMs及10 g/L的黑糖，其水萃取物ACEi的乾重、水溶性蛋白及胜肽其IC50值，分別約為0.98, 0.12及0.13 mg/mL。然而經發酵12天後，其水萃取物ACEi的乾重、水溶性蛋白及胜肽其IC50值，分別約為0.85, 0.10及0.10 mg/mL。此項研究有助於利用脫脂黑芝麻粕以牛樟芝發酵來開發具抗高血壓活性之保健產品。

關鍵字

脫脂黑芝麻粕 ； 牛樟芝 ； 發酵 ； 水萃取物 ； 血管緊張素轉化酶抑制活性

並列摘要

This study is to investigate the ACE inhibitory activity (ACEi) of water extracts (WEts) from 50g/L defatted black sesame meals (DBSMs) with 10g/L brown sugar fermented by Antrodia camphorata, a “ruby in mushroom” present from heaven for Taiwanese, in submerged cultivation at 28oC for 14 days. The ACEi of WEts from fermented DBSMs (fmtedDBSMs) was higher than that of WEts from unfermented DBSMs (unfmtedDBSMs). The effect of extraction ratio (1:200, 1: 100, 1:50, 1:10, 1:5 defined as 1 g freeze-dried DBSMs extracted with 200, 100, 50, 10, 5 mL water, respectively) on ACEi of WEts was also studied. The ACEi of WEts was increased with the increase of extraction ratio. In addition, the ACEi and the soluble protein and peptide contents were decreased with increasing the dilution fold of WEts. Furthermore, the ACEi percentage (ACEi%) was increased up to the saturation value (around 88 %) with increasing the soluble protein and peptide contents of WEts. This indicated the soluble protein and peptide released during DBSMs fermentation could contribute to the ACEi. However, the ACEi expressed in terms of IC50 values were about 0.99, 0.19 and 0.14 mg/mL for dry weight, soluble protein and peptide concentrations of WEts, respectively. For the medium with 10g/L DBSMs and 10g/L brown sugar, the IC50 values were about 0.98, 0.12 and 0.13 mg/mL for dry weight, soluble protein and peptide concentrations of WEts, respectively. However, the IC50 values for 12 days of fermentation time were about 0.85, 0.10 and 0.10 mg/mL for dry weight, soluble protein and peptide concentrations of Wets, respectively. These findings will contribute to the development of the fmtedDBSMs by A. camphorata to new health-food products with anti-hypertensive activity.

並列關鍵字

defatted black sesame meals ； Antrodia camphorata ； fermentation ； water extracts ； ACE inhibitory activity (ACEi)

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