- 學位論文
利用單光束光鉗量測病變白血球之交互作用力
Measurements of The Interactive Force Between Jurkat Cells Using a Single-Beam Optical Tweezer
摘要
本文先介紹光鉗的工作原理及如何求出光鉗的鉗住力並將其應用在細胞作用力之量測。本研究是利用一單光束光鉗，首先捕捉物是直徑3.0 μm大小的聚苯乙烯微粒，完成鉗住微粒測試後改成人類病變白血球細胞株Jurkat 細胞的操縱。本實驗的光鉗雷射波長有532 nm和671 nm兩種波長，由於可見光波長雷射對細胞會具有傷害性，所以使用較低功率去捕捉細胞實驗，先分別測量兩個或三個Jurkat細胞在不同的黏住時間下，所需分離的力量，實驗顯示兩個細胞黏住時間越久，要分離其中一個細胞的力量也就越大，而三個細胞黏住的力量又比兩個細胞黏住時大一些，細胞間黏附力量與黏附時間是呈現線性增加關係。在鉗住細胞互黏時，其操縱細胞的功率大小也會影響Jurkat細胞間黏住的長度大小，實驗顯示當操縱相互黏住時功率越大，細胞間黏住輪廓邊界越長，而欲分離兩個黏附細胞的力量也就越大。當Jurkat細胞欲與聚二甲基矽氧烷(PDMS)材料黏附時，其細胞與PDMS黏附時間需要比細胞與細胞黏附時間更長才能較穩定黏住，實驗顯示細胞與PDMS黏附時間越久，分離力量也就越大，其黏附力與黏附時間呈現增加關係，而且細胞之間的黏附則較細胞與PMDS黏附力量大一些。
並列摘要
This paper presents a single beam optical tweezers used to measure the interactive force between Jurkat cells (the diseased white blood cells with a diameter of 14.0 μm). Optical trapping of beads and Jurkat cells using a single-beam optical tweezer have been achieved. The experimental results show that a laser power of 2.4 mW is sufficient to trap 3-μm-diameter polystyrene beads, while a laser power of 1.5 mW is sufficient to trap individual Jurkat cells. Since the laser (λ=532 nm) can cause large damage when used in biological manipulation. Therefore, the Jurkat cells were trapped using a laser (λ=671 nm) with a low laser power condition. Separating two mutually attached Jurkat cells requires a laser power of 4 mW. Experimental results show that as the adhesion time of the two Jurkat cells is increased, the separation power also increases. Compared with two mutually attached Jurkat cells, three mutually attached Jurkat cells require a large separation power. The Jurkat cells adhered to the PDMS structure has also been investigated. Experimental indicate that as the adhesion time is increased between the Jurkat cells and the PDMS structure, the separation power also increases.