- 學位論文
市售不同蜂花粉產品之抗氧化活性特性分析
Antioxidant Properties of Different Bee Pollen Products
摘要
隨著保健養生的意識抬頭，人們於利用蜜蜂相關產品的基礎上，逐漸地瞭解且接觸並開始應用蜂花粉。蜂花粉中的多酚及黃酮類化合物提供了良好的抗氧化活性，對人類的健康有很大的益處，可減少人體被自由基攻擊。蜂花粉的成分及香氣亦會因花粉來源不同而有很大的差異，本研究將針對常見的幾種蜂花粉產品進行抗氧化活性測試並比較之間的差異性。在此分別針對油菜花粉、百草花粉、蒲鹽花粉、茶花粉、松花粉、花粉錠、含羞草花粉、混合型花粉、多種花粉源花粉等九種不同蜂花粉產品進行抗氧化測試包含清除DPPH自由基能力、清除氫氧自由基能力、清除超氧自由基能力、抑制亞麻油酸能力及還原力等抗氧化活性方法測試，並分析其總蛋白質含量以及利用磷鉬酸酚試劑（Folin-Ciocalteu reagent）分析法量測其總酚類含量。結果顯示，不同蜂花粉產品因花粉源及製程或加工不同，總酚類含量即不同，進而影響了抗氧化活性之表現。其中以油菜花粉、百草花粉、蒲鹽花粉及花粉錠於多項抗氧化能力測定下，具有較佳之抗氧化性效能；而多種花粉源花粉產品的抗氧化性能力則較其他蜂花粉產品差。
並列摘要
This study aimed to evaluate the antioxidant properties of different bee pollen products. Nine samples were collected by Fu-Chang Beekeeping in Hualien, Taiwan or bought from the market of Taiwan. Rape pollen product displayed predominant antioxidant properties, including scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and inhibition of linoleic acid peroxidation. However, lower antioxidant activities were observed in the mixed bee pollen product for the assay of DPPH radicals, inhibition of linoleic acid peroxidation, scavenging activity of superoxide anion radicals and reducing power. In addition, higher contents of proteins were determined in the pollen tablet product and the higher contents of polyphenolic compounds were determined in the wild flower pollen product than others. Many reserch demonstrated that the polyphenolic compounds may be the major component for giving the antioxidant activities in bee pollens. In summary, the bee pollen products from differrent plant species and via processed products did affect the antioxidant potencies, and rape pollen product showed the most substantial antioxidant activities.