- 學位論文
多孔性高分子水膠之葡萄糖濃度檢測晶片研製
Development of the Porous Hydrogel-Based Microchip for Glucose Detection
摘要
糖尿病患者的血液與尿液中葡萄糖含量高於一般人。因此血糖和尿糖中葡萄糖的篩檢就成為了監控糖尿病主要方法。在現今的檢測方法中分成以抽取患者血液檢測血糖的侵入式技術與檢測尿液中的尿糖或皮膚的熱量代謝等非侵入式技術兩大類，但由於抽血容易造成患者的排斥以及各種非侵入式裝置存在著檢測上易產生誤差與不方便等缺點，因此本研究目的主要是以非侵入式為前提，藉由酵素與葡萄糖產生反應變色的特性，設計篩檢晶片達到快速與準確的葡萄糖濃度檢測。主要為利用黃光微影製程技術並整合化學表面修飾技術，於晶片上以混合葡萄糖酵素、過氧化酶與碘化鉀之水膠製做出柱狀結構，並以不同濃度之葡萄糖溶液進行測試。在研究中利用葡萄糖酵素對葡萄糖產生反應，所得產物中含有過氧化氫，其經由過氧化酶催化促使其氧化碘化鉀，並使得碘化鉀產生顏色。在實驗中並同時以影像分析軟體ImageJ進行呈色上的分析，以求得到精確的呈色強度值，並確定此方法的趨勢與正確性。研究結果顯示高濃度到低濃度葡萄糖於結構上會產生紅棕色、青色與藍色，此外為加強低濃度的呈色判別，嘗試以新的呈色劑4-AAP與TBHBA做研究，藉此判斷出溶液所含之葡萄糖濃度，達到葡萄糖篩檢的目的。
並列摘要
The diabetics’ blood and urine glucose levels are higher than normal people; therefore, glucose test in blood or urine has become the main method for monitoring diabetes. The methods of the include detecting the invasive technique that drawing blood from patients, and the non-invasive technique that detecting glucose level in urine or metabolism heat of skin. Because of the facts that exsanguinating is uncomfortable for patients as well as a variety of error and inconvenience for non-invasive devices, The main purpose of this study is based on non-invasive devices employing enzyme produced reactions with glucose to design chips and achieve rapid and accurate sensing for glucose concentrations. A hydrogel-based microchip was developed to achieve the the glucose concentration detection. The proposed chip was fabricated using standard photolithography technique integrating with the chemical surface modifications. The glucose enzyme was mixed with the hydrogel to detect various concentrations of the glucose solutions. The glucose enzyme reacts with glucose, and showed the color in the structure herein. In the experiment, colors were analyzed by ImageJ software, in order to obtain an accurate value of color intensity, and identify trends and accuracy of this method. The results showed that high to low concentrations of glucose will produce a reddish brown in the structure, green and blue. In addition to strengthening the determination of low concentrations respectively new chromogenic agent of the 4-AAP and the TBHBA were investigated. Experimental results indicated the glucose solutions with different concentrations revealed various color in the chip; hence, the concentration of glucose could be determined.