摘要
豬生殖與呼吸綜合症（PRRS）是豬全世界經濟最重要的疾病。典型的PRRSV感染豬的免疫功能是遲發性和低水平的中和抗體反應及弱的細胞介導的免疫反應。本病主要引起豬隻早產，流產，死胎，木乃伊胎，重症肺炎，水腫及結膜炎。基因組是長度約15 KB，其中包含9個ORFs。一些其他因素，如動物的年齡和細菌混合感染能夠影響病毒的複製和臨床症狀表現。產氣莢膜梭狀芽胞桿菌是人類和牲畜的主要病原菌。產氣莢膜梭菌腸毒素（CPE）會導致幾種常見的消化道疾病相關的症狀。文獻指出，經由皮下注射免疫小鼠CPE 融合蛋白可活化特異性黏膜免疫反應及全身性黏膜反應。M和GP5蛋白在引發中和性抗體上是必需的，因此我們建構M -GP5 -CPE 融合蛋白，利用原核表現系統表現之，以SDS-APGE及Western blotting 確認產物大小及其抗原性，經皮下注射免疫動物實驗後利用ELISA、細胞激素測定與中和抗體試驗來評估疫苗效力。
並列摘要
Porcine reproductive and respiratory syndrome virus (PRRSV) is the most economically significant disease of swine worldwide. Typical immunological features in PRRSV-infected pigs are delayed onset and low level of neutralizing antibodies and a weak cell-mediated immune response. The disease mainly causes premature delivery, miscarriage, stillbirth, mummified fetuses, severe pneumonia, edema and conjunctivitis in pigs. PRRSV belongs to the family Arteriviridae in the order Nidovirales, a family of positive-sense, single stranded linear RNA viruses. The genome is about 15 kb in length which contains nine ORFs. Several other factors such as animal age and bacterial co-infection can influence virus replication and clinical signs. Clostridium perfringens is a major pathogen of humans and livestock. Clostridium perfringens enterotoxin (CPE) causes the symptoms associated with several common gastrointestinal diseases. It's found that subcutaneous immunization of mice with the CPE fusion protein activated antigen-specific mucosal and systemic immune responses. M and GP5 proteins are essential for the neutralizing antibody, so we constructed a M -GP5 -CPE fusion proteins in prokaryotic expression system. The molecular weight and antigenicity were confirmed by SDS-PAGE and Western blot. Then, the subunit vaccine efficacy will be revealed by animal testing with intranasally immunized. Moreover, the immunoassay were tested by ELISA and determination of cytokine and neutralizing antibodies testing.