摘要
日本須見洋行教授於1982年證實納豆中含有溶纖維蛋白酶，並將其命名為納豆激酶（nattokinase）。納豆激酶主要功用為分解血栓、預防因血栓所引起的腦中風、心肌梗塞等心血管疾病；納豆激酶具有很強的纖維蛋白溶解能力，並具有影響凝血塊之生成及調節多種纖維蛋白代謝之能力。如將納豆及納豆萃取液應用於人工血栓物質（纖維蛋白平板），結果可以發現放置於纖維蛋白平板表面之納豆其周圍會產生一圈透明的溶血環，代表納豆具有溶解血栓的能力。本實驗預計將分二階段進行，第一階段為納豆激酶萃取與纖維蛋白平板測試，從市售的納豆產品中萃取出納豆激酶，並將萃取出的納豆激酶投入纖維蛋白平板，觀察實際的溶血情形。第二階段為動物實驗，準備紐西蘭白兔為實驗動物並製造人工血栓，製造出來後投與納豆激酶來觀察其在體內的溶血栓情形且做時間的記錄，以血壓計監測製造血栓前後血壓的變化，再用病理切片觀察人工血栓是否形成。纖維蛋白平板結果顯示，在2、4、6、8小時之溶血環平均寬度分別為0.7、0.9、1.2、1.3mm；且口服納豆菌液前後記錄凝血時間利用SAS系統之T-TEST統計也具有顯著性差異（P＜0.005），表示納豆激酶具有溶解血栓、延長凝血時間之效力。利用40％氯化鐵製造出之人工血栓具有最佳之阻塞效果，在口服納豆激酶後觀察血管變化，顯示血管內阻塞之人工血栓有被溶解現象，且血壓與只被氯化鐵傷害時之血壓變化相較下相當穩定，利用ANOVA做三重複分析顯示有顯著性差異（P＜0.005），證明納豆激酶具有穩定血壓、溶血栓之效果。
並列摘要
In 1982, Japanese professor Hiroyuki Sumi had proved that Natto contains fibroproteinase and named it as nattokinase. The main function of nattokinase is to decompose blood clots and to prevent cardiovascular diseases caused by thrombosis, such as cerebral hemorrhage, or myocardial infarction, nattokinase possesses the powerful ability of dissolving fibrin and of the influence on fabrication of hemagglutinin and regulation at plenty kinds of fibrin metabolism. If we put natto and its extract on artificial thrombosis material experiments (fibrin slab), the surface of fibrin slab will display limpid hemolytic ring surrounding natto, which means that natto have the ability of dissolving thrombosis. The experiment is divided into two trials. The first trial is to extract nattokinase from the commercially available products and put the extracted product on fibrin plate to observe the actual situation of hemolysis. The second trial is animal experiment with New Zealand white rabbits as animal model, and to create artificial thrombus,then to observe nattokinase hemolysis in vivo situation, and monitor the blood pressure changes before and after the production of blood clots.Furthermore, the formation of artificial thrombus is studied pathologically. Fibrin plate results showed that the average diameters of hemolysing ring 2,4,6 and 8 hours after being exposed to natto bacilli were 0.7,0.9,1.2 and 1.3mm, respectively. In addition, the recorded clotting time before and after the oral administration of natto bacilli showed significance (P<0.005) in T-TEST using SAS statistical system, indicating that nattokinase is capable of dissolving thrombus and extending blood cotting time. The artificial thrombus induced by 40% FeCl3 has the greatest effect on vascular obstruction, observing vascular change post to oral administration of nattokinase showed that the artificially induced thrombus has been dissolved, and its blood pressure is relatively more stable comparing to that modified with only ferric chloride. Using ANOVA triple analysis showed significance (P<0.005) proving that nattokinase could stabilize blood pressure and dissolve thrombus.