微脂體包覆薑黃素之安定性及抗氧化活性評估

文獻指出微脂體包覆活性物質可增進其生物利用率，已知薑黃萃取薑黃素為脂溶性多酚類物質，本研究以精鍊磷脂質搭配薄膜水合法，製備搭載薑黃素的微脂體，利用加速性試驗、紫外光破壞試驗以及細胞培養試驗，探討不同均質條件製備微脂體安定性以及抗氧化活性。結果顯示，鐳射粒度分布儀測量微脂體粒徑與粒徑分布，在儲存 28天後，低轉速及中轉速平均粒徑變動不大，又以高轉速呈現較不穩定，而在不同劑量的UVB照射下，則因劑量提升造成粒徑變大趨勢。在細胞毒性試驗中，細胞在不同濃度薑黃素經處理24、48 小時，IC50值濃度為 177.7ug/ml、 59.49ug/ml，在不同濃度薑黃素的微脂體，IC50 值濃度為 250.4ug/ml、 80.93ug/ml，兩者具有顯著差異，顯示出微脂體包覆薑黃素後有利於細胞提高細胞存活率。在活性氧測試中，與對照組 H2O2 比較，添加 H2O2 分別與薑黃素及搭載薑黃素的微脂體共同處理，ROS 生成量明顯下降很多，有助於減緩 H2O2 的氧化壓力達到保護細胞效果。

關鍵字

微脂體；薑黃；抗氧化活性； UVB

並列摘要

Research indicates that the liposomes systems as a carrier can improve the biological efficiency. It is known that curcumin extracted from turmeric is a fat-soluble polyphenolic substance, in this study, the thin-film hydration method was used to encapsulating curcumin by refined phospholipids to liposomes and the method was conducted to evaluate the stability of the liposomes and the activity of antioxidant activity by an accelerated test, UVB irradiation and cell culture assay. The instrument of laser diffraction particle size analyzer was used to measure the particles size and the distribution of the liposomes. After 28 days of storage, the average particle size showed insignificant differences between low spinning speed and medium spinning speed, while unstable particle size showed under high spinning speed. However, the particle size showed a dose dependent UVB irradiation that it will decrease in higher UVB irradiation. In cytotoxicity assay, the concentration of IC50 was 177.7 ug/ml and 59.49 ug/ml when the cells were treated with different concentrations of curcumin for 24 and 48 hours, and the concentrations of curcumin loading liposomes in different concentrations were 250.4 ug/ml and 80.93 ug/ml. As a result of the above, the significant differences between curcumin and curcumin-loaded liposomes elucidated that, it is beneficial for cells to increase cell viability after liposomes are coated with curcumin. In the reactive oxygen species assay, they were co-treated with curcumin and curcumin-loaded liposomes. The amount of ROS production was significantly reduced, which helped to reduce the oxidation pressure to protect cell. In the reactive oxygen species (ROS) assay, the co-treatment between H2O2 with curcumin and curcumin-loaded liposomes demonstrated a significant reduced on the amount of ROS production compare to the control. This result explained that curcumin and curcumin-loaded liposomes able to protect the cells from the oxidation stress.