不同餌料、溫度和鹽度對吻海馬成長表現和抗氧化活性之影響

Effects of food type, temperature and salinity on the growth performance and antioxidant status of the longsnout seahorse, Hippocampus reidi

余仁愛(Yu Jen-Ai)

指導教授 ： 劉俊宏

國立屏東科技大學/農學院/水產養殖系所/碩士(2020年)

https://doi.org/10.6346/NPUST202000409

摘要

本實驗在評估餌料、鹽度和溫度對吻海馬(Hippocampus reidi)幼魚的成長和抗氧化能力的影響。實驗分為3個試驗，且分別進行56天的實驗。結果顯示餵飼冷凍糠蝦 (MD)組的存活率、成長表現與飼料效益顯著較餵飼冷凍豐年蝦成蟲 (AD)或由MD和AD以1：1(w/w)混合 (MAD)餵飼的海馬好。飼養在30℃的海馬飼養至第49天時，死亡率達到100％。飼養在26℃組的海馬的存活率和成長表現較飼養在28℃組的高，但無統計上差異。當鹽度15-20 ppt時，海馬有較好的成長表現，但飼養在不同鹽度組之間的海馬的存活率和生長表現並無顯著差異。根據總酚含量（TPC）和清除1,1-二苯基-2-三硝基苯肼（DPPH）自由基的能力的結果顯示，MD組和MAD組的海馬具有較佳清除自由基的抗氧化能力，且MD組海馬的還原能力明顯較AD組海馬高。而飼養在26℃或28℃組的海馬的各種抗氧化能力指標分析結果均無顯著差異。飼養在15 ppt和20 ppt組的海馬的TPC、DPPH自由基清除活性和還原性鐵還原抗氧化能力活性顯著較飼養在較高鹽度組的海馬高，且海馬飼養在15 ppt組的金屬螯合活性顯著高於在其他高鹽度組。根據上述的結果，吻海馬幼魚的建議飼養條件為溫度26℃、鹽度15-20 ppt之環境且以冷凍糠蝦為飼養餌料。

關鍵字

吻海馬 ； 餌料 ； 環境條件 ； 成長 ； 抗氧化能力

並列摘要

The effects of food type, salinity and temperature on the growth performance and antioxidant status of longsnout seahorse juveniles, Hippocampus reidi, were evaluated in this study. Three trials were carried out for 56 days. Seahorses fed frozen mysid shrimp (MD) exhibited significantly higher survival, growth performance, and feed efficiency compared to that of seahorses fed frozen adult Artemia salina (AD) and a mixture consisting of MD and AD at the ratio of 1:1 (MAD). Mortality of seahorse cultured at 30oC reached 100% at 49 days. Despite no significant difference, the survival and growth performance of seahorses were higher at 26oC than 28oC. Seahorses had better growth performance at salinities of 15-20 ppt, but the survival and growth performance of seahorses were not significantly different among salinity groups. Seahorses fed MD and MAD had better antioxidant status based on the results of total phenolic content (TPC) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity in the MD and MAD groups, and significantly higher reducing power in MD than AD. No significant difference in all antioxidant indicators was recorded in 26oC and 28oC groups. TPC, DPPH radical scavenging activity, and ferric reducing antioxidant power activity of seahorses were significantly higher at 15 ppt and 20 ppt, and metal chelating activity of seahorses at 15 ppt was significantly higher than that of seahorses at higher salinities. Based on these results, H. reidi juvenile is recommended to be fed mysid shrimp at a temperature of 26 oC and salinity of 15-20 ppt.

並列關鍵字

Hippocampus reidi ； growth out ； food ； environmental condition ； antioxidant status

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