- 學位論文
乳牛分娩前果糖胺及圍產期糖化白蛋白濃度與產後的代謝狀態、卵巢功能及乳量之關係
The Association of Prepartum Fructosamine and Peripartum Glycated Albumin with Metabolic Status, Ovarian Function and Milk Yield in Postpartum Dairy Cows
摘要
果糖胺 (Fructosamine, Fruc)和糖化白蛋白 (Glycated albumin, GA)是血清中的糖化蛋白質，在人類和小動物可反映過去血糖 (Glu)濃度。先前的研究顯示，乳牛圍產期的血糖濃度與圍產期疾病、營養狀態和生殖表現有關。本研究的目的是評估乳牛分娩前的Fruc和圍產期間的GA濃度，並探討它們與代謝狀態、生殖表現和乳量的關係。本研究使用26頭健康的經產荷仕登乳牛，在預產期10天前(-10d)檢測血中Fruc與GA濃度，並於-10d以及分娩後7、14、21和28天(7d, 14d, 21d and 28d)採集血液樣本，測定Glu、白蛋白 (Alb)、球蛋白 (Glb)、總蛋白 (TP)、γ-谷氨酰轉移酶 (GGT)、天冬氨酸轉氨酶 (AST)、膽固醇 (Cho)、血中尿素氮 (BUN)、鈣 (Ca)、鎂 (Mg)、β-羥基丁酸 (BHBA)和非酯化脂肪酸 (NEFA)的濃度，28d的血液樣本同時檢測GA濃度，並記錄乳牛產後21天內是否有排卵以及分娩後第30天之乳量。結果顯示乳牛分娩前GA及Fruc兩者可能與肝臟氧化壓力有關，其中GA與能量平衡有關。分娩前的Fruc與泌乳初期的乳量表現呈正相關。乳牛分娩後的GA的上升可能與炎症、肝臟氧化壓力以及營養狀態較差有關。在相關性分析中，GA與過去一至二週的Glu呈現中等至高度的正相關。此外，在分娩後有早期排卵的乳牛，-10d GA有較低的趨勢 (p = 0.06)。根據結果，乳牛分娩前的Fruc和圍產期間的GA濃度可能有潛力做為泌乳初期代謝狀態、生殖表現和乳量的指標。
並列摘要
Fructosamine (Fruc) and glycated albumin (GA) are serum glycated proteins that reflect the retrospective glucose (Glu) concentrations in humans and small animals. Previous studies have shown that peripartum Glu concentrations related to peripartum diseases, nutritional status and reproductive performance in dairy cows. The aim of this study was to evaluate prepartum Fruc and peripartum GA in dairy cows and to investigate their association with metabolic status, reproductive performance and milk yield. Twenty-six healthy multiparous Holstein cows were enrolled into the study. Blood samples were collected at -10, 7, 14, 21 and 28 days relative to calving (-10d, 7d, 14d, 21d and 28d). The Fruc was measured at -10d, GA was mearsured at -10d and 28d. The Glu, albumin (Alb), globulin (Glb), total protein (TP), gamma-glutamyl transferase (GGT), Aspartate aminotransferase (AST), cholesterol (Cho), blood urea nitrogen (BUN), calcium (Ca), magnesium (Mg), β-hydroxybutyrate (BHBA) and non-esterified fatty acids (NEFA) were measured at -10d, 7d, 14d, 21d and 28d. Ovulation within 21 days after calving and milk yield at 30-day in milk were recorded in this study. The results showed that prepartum GA and Fruc may be related to liver oxidative stress, and GA is related to energy balance. Prepartum Fruc was positively correlated with milk yield in early lactation. The increase of GA after calving may be related to inflammation, liver oxidative stress and poor nutritional status. In the correlation analysis, GA showed a moderate to strong positive correlation with Glu in the past one to two weeks. Moreover, the cows that ovulated within 21 days after calving had a lower tendency of GA (p = 0.06). The results indicated that prepartum Fruc and peripartum GA in dairy cows may be potential indicators of metabolic status, reproductive performance and milk yield in the early stages of lactation.