- 學位論文
食品中多酚含量快速檢測技術之開發
Development of rapid detection technigue for polyphenol content in foods
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摘要
多酚 (Polyphenol)是一個天然存在的有機化合物大家族，其特徵是由苯酚單元所組成，具有豐富的結構與多樣性。多酚家族中存在許多種結構變體，大多數是在水果和蔬菜中發現的生物活性化合物。目前常見檢測多酚之方式是以分光光度法為主，但需要分析的時間較長，因此對於快速便捷且更經濟化分析方法之需求日益增加。首先利用Folin-Ciocalteu的方法進行反應產生藍綠色化合物，以分光光度法在750 nm之波長下測定，於濃度0-500 ppm間得到良好的線性迴歸式，y = 0.0017x + 0.1243，R² = 0.9982。反應時間於90分鐘後可以得到穩定且高重現性之結果，利用此方法進行真實樣品測定-回添試驗，結果顯示會因為樣品本身的顏色影響測定的結果。開發搭配3D列印技術製造之微流體紙基分析裝置 (microfluidic paper-based device, μPAD)，該裝置使用光固化樹脂作為邊界疏水材料，可更快速製造μPAD與檢測食品中多酚含量，以修正過的Folin-Ciocalteu試劑在微流體紙基上進行反應，在不同的濃度 0-680 ppm間，使用色階軟體ImageJ分析RGB值，得到良好的線性迴歸式，y = 0.0022x + 6.5136，R² = 0.9263。反應10分鐘後即可進行分析，可以得到穩定且高重現性之結果。利用此方法進行真實樣品測定，發現微流體紙基分析法會高估樣品本身的濃度，無法測得真實濃度。本研究所開發微流體紙基分析法並不適用於定量多酚含量，但其優點可以用來初篩未知植物的多酚含量，初步定量多酚含量的高低，快速判別後以利進行後續分析。
並列摘要
Polyphenols are a large family of naturally occurring organic compounds characterized by a rich structural diversity of phenolic units. There are many structural variants in the polyphenol family, most of which are bioactive compounds found in fruits and vegetables. Currently, spectrophotometry is the most common method for the determination of polyphenols, but it takes a long time to analyze them, so there is an increasing demand for fast, convenient, and more economical analytical methods. The Folin-Ciocalteu method was used to produce the blue-green compound, which was measured spectrophotometrically at 750 nm, and a good linear regression was obtained in the concentration range of 0-500 ppm, with y = 0.0017x + 0.1243, and R² =0.9982. Stable and highly reproducible results were obtained after a reaction time of 90 min. The results of the real sample measurement and spike test using this method, show that the color of the sample itself affects the measurement results. A microfluidic paper-based device (μPAD) was developed with 3D printing technology, which uses light-curing resin as the boundary hydrophobic material to fabricate μPADs and detect polyphenol content in food products more rapidly. The RGB values were analyzed using the color gradient software ImageJ, and a good linear regression was obtained, y = 0.0022x + 6.5136，R² = 0.9263. The analysis was completed after 10 minutes of reaction. Stable and highly reproducible results can be obtained using this method for real sample determination. The microfluidic paper-based analysis method was found to overestimate the concentration of the sample itself, and the true concentration cannot be measured. The microfluidic paper-based analysis developed in this study is not suitable for quantification of polyphenol content, but it has the advantage of being used for initial screening of unknown plants for polyphenol content, characterization of polyphenol content, and rapid identification for subsequent analysis.
參考文獻
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