- 學位論文
台灣2020-2023年間具致病性赤痢螺旋體菌之檢測
Detection of pathogenic Brachyspira species in Taiwan during 2020-2023
摘要
豬赤痢 (Swine dysentery, SD) 為豬隻重要腸道疾病之一，此病好發於保育期及肥育期豬隻，其臨床症狀及病變包括消瘦、血痢及黏液性出血性結腸炎，影響豬隻的飼料換肉率，亦會造成豬隻死亡，令養豬戶蒙受嚴重的經濟損失。在北美洲、歐洲及亞洲多個國家皆曾檢測出豬赤痢，然而針對豬赤痢盛行率之調查依然缺乏相關之詳細資料。據目前之研究所知，豬赤痢的病原主要為Brachyspira sp. 內具強β溶血性的B. hyodysenteriae、B. hampsonii、B. suanatina，其主要診斷方式為細菌分離、聚合酶連鎖反應、及原位雜合染色。然而，台灣地區針對豬赤痢病原之檢測相關研究相當有限，並且亦無能夠同時檢測出上述3種病原及B. pilosicoli的聚合酶連鎖反應檢測技術 (polymerase chain reaction, PCR)，故本研究目的欲引用新建立之能夠同時檢測出以上4種具致病性Brachyspira sp.的多引子對聚合酶連鎖反應檢測技術 (multiplex quantitative real-time PCR, multi-qPCR)，並了解B. hyodysenteriae及B. pilosicoli於2020-2023年之檢出率及台灣地區內是否有B. hampsonii及B. suanatina存在。本研究中自2020-2023年期間共收集到878例樣本，且在後續進行multiplex qPCR。本研究中利用multiplex qPCR所得之B. hyodysenteriae、B. pilosicoli、B. suanatina及B. hampsonii的檢出率分別為21.9% (192/878)、2.2% (19/878)、0.6% (5/878) 及0% (0/878)。本次研究亦確定台灣地區內有B. suanatina之出現。
並列摘要
Swine dysentery (SD) is a major enteric disease in swine, it is most often observed in grower-finisher pig. The most significant clinical signs and gross lesion of this disease included loss of body condition, bloody diarrhea and mucohemorrhagic colitis, which cause poor feed conversion and mortality of pigs. Therefore, the occurrence of this disease will cause a severe financial impact to the affected farm. Furthermore, there were several researches had pointed out SD had been detected in multiple countries in North America, Europe, and Asia. However, the detailed information about prevalence of SD in different countries is still limited. Refer to the previous researches, the possible pathogens that mainly causing SD are B. hyodysenteriae, B. hampsonii, B. suanatina from Brachyspira genus with strongly β-haemolytic characteristic, and the diagnostic methods of SD included microbial isolation, polymerase chain reaction (PCR) and in situ hybridization. However, in Taiwan, the studies about the detection of etiologic agents of SD are still limited, and there is also missing of a PCR technique which can simultaneously detect the 3 pathogens mentioned above and B. pilosicoli. Therefore, the purposes of this study are: i) to modify a newly developed multiplex quantitative real-time PCR (multi-qPCR) technique which then can simultaneously detect the 4 pathogens mentioned above, ii) to investigate the detection rate of B. hyodysenteriae and B. pilosicoli, iii) to determine the existence of B. hampsonii and B. suanatina in Taiwan. In this study, total 878 samples were collected during year 2020-2023, these samples were then advanced to multi-qPCR examination. The total detection rates of B. hyodysenteriae, B. pilosicoli, B. suanatina, and B. hampsonii by using multi-qPCR are 21.9% (192/878), 2.2% (19/878), 0.6% (5/878) and 0% (0/878), respectively. This study is also determined the existence of B. suanatina in Taiwan.