- 學位論文
研究台灣香檬精油淡斑的效果
The effect of Citrus depressa Hayata essential oil on treating skin hyperpigmentation
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摘要
台灣香檬(Citrus depressa Hayata)是台灣原生種植物，在日本沖繩地區是有名的保健食物，並根據當地喜好育種出多種品系。香檬汁有多種功效，而香檬榨完之後剩下的果肉果皮仍含有豐富的精油可利用。本研究分析台灣香檬精油是否有淡化皮膚斑點的效果。方法是以小鼠黑色素瘤細胞株B16F10及角質生成細胞株HaCaT為模型，分析精油是否能促進黑色素降解及是否能抑制黑色素生成。在結果發現，香檬精油不能直接降解黑色素，但是可以促進人類皮膚角質生成細胞株降解黑色素。此外，香檬精油能有效抑制小鼠黑色素瘤細胞株B16F10的黑色素合成，但不能抑制其分泌；美白標準品熊果素不能抑制黑色素合成，但能抑制其分泌。結果並證明，香檬精油是酪胺酸酶的抑制劑，因此，香檬精油可促進皮膚細胞降解黑色素，又能抑制黑色素的生合成，推測其具有淡化黑斑的效果。
並列摘要
C. depressa belong to the citrus species. In this study, we tried to analyze whether C. depressa essential oil possesses similar skin-lightening effects. The mouse melanoma cell line (B16F10) and human keratinocyte cell line (HaCaT) were used as models to analyze whether essential oils can promote cells to produce melanosomes or degrade melanin. In the result, C. depressa essential oil did not directly degrade melanin. However, it could promote melanin degradation by human keratinocytes. Moreover, C. depressa essential oil effectively inhibited melanin synthesis in the B16F10 melanoma line but did not inhibit its secretion. In contrast, the whitening agent arbutin did not inhibit melanin synthesis but could suppress its secretion. The results also demonstrate that C. depressa essential oil acted as an inhibitor of tyrosinase. Therefore, C. depressa essential oil can promote melanin degradation by keratinocyte while inhibition the biosynthesis of melanin, suggesting that it may have a lightening effect on hyperpigmentation.
參考文獻
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