- 學位論文
牛樟芝純化物在放射線治療下保護正常細胞效果
The purified Antrodia camphorata protects normal cells under radiation therapy
摘要
癌症是全球性的健康問題，是僅次於心血管疾病成為全球第二大死因，占全球疾病死亡人數的六分之一，在癌症眾多治療方式中最普遍被使用的就是放射治療，根據臨床統計，70%的癌症患者需要放射治療，而其中約有40%的癌症可以透過放射治療成功治癒。放射治療是透過電離輻射直接誘導DNA中的單鏈斷裂和雙鏈斷裂，導致細胞分裂和增殖停止以及細胞壞死和凋亡，達到破壞癌細胞和腫瘤組織的目的。放射治療在破壞癌細胞的同時也會破壞正常細胞，一個放射治療的完整療程通常包括20至40次以上的放射治療，如果在過程中正常細胞無法順利恢復，放射治療的副作用就會出現。放射治療的副作用可以分為急性、亞急性、慢性，急性副作用大部分都是可逆的而慢性副作用則大部分不可逆，在放射治療中不管是對癌症的治療效果還是副作用發生的嚴重程度都與放射劑量息息相關。 牛樟芝是一種原產於台灣的真菌，具有相當多藥理活性，其本身具有抗氧化和抗炎作用，在許多有關牛樟芝的研究中表明，牛樟芝能夠有效抑制細胞毒性，許多實驗證明在輻射引起的組織損傷中，牛樟芝可以抑制免疫調節訊號，以避免體外免疫細胞的細胞毒性和發炎反應，同時牛樟芝的研究中還有與多種化療藥物合併使用，能夠減少化學治療藥物使用劑量並且降低化學治療帶來的副作用， 本實驗的目的在於透過體外培養人類肺成纖維細胞IMR-90，來測試由元樟生技股份有限公司提供的三種牛樟芝純化物是否可以保護正在接受放射治療的正常人體細胞並促進後續的恢復，探討牛樟芝除了與化學治療相結合，是否也能在放射治療時成為天然的放射保護劑。而在實驗中成功證實三種牛樟芝純化物中，其中一種對於維持細胞存活率有幫助，以及相較於在放射治療後給藥，選擇在接受放射治療前給予IMR-90細胞牛樟芝純化物YUAN01是更有效的。
並列摘要
Cancer is a global health problem. It is the second leading cause of death in the world after cardiovascular disease, accounting for one-sixth of global disease deaths. Among the many cancer treatments, radiotherapy is the most commonly used. According to clinical Statistics show that 70% of cancer patients require radiation therapy, and about 40% of these cancers can be successfully cured through radiation therapy. Radiotherapy uses ionizing radiation to directly induce single-strand breaks (SSB) and double-strand breaks (DSB) in DNA, leading to the cessation of cell division and proliferation, as well as cell necrosis and apoptosis, thereby destroying cancer cells and tumor tissues. Radiation therapy destroys cancer cells as well as normal cells. A complete course of radiation therapy usually includes more than 20 to 40 times of radiation therapy. If normal cells cannot recover smoothly during the process, side effects of radiation therapy will appear. The side effects of radiotherapy can be divided into acute, subacute, and chronic. Most of the acute side effects are reversible, while most of the chronic side effects are irreversible. In radiotherapy, both the therapeutic effect on cancer and the severity of side effects are closely related to radiation. Dosage is closely related. Antrodia camphorata is a fungus native to Taiwan that has considerable pharmacological activity. It has antioxidant and anti-inflammatory effects. Many studies on Antrodia camphorata have shown that Antrodia camphorata can effectively inhibit cytotoxicity. Many experiments have proven that Antrodia camphorata can effectively inhibit cytotoxicity in radiation-induced During tissue damage, Antrodia camphorata can inhibit immune regulatory signals to avoid cytotoxicity and inflammatory reactions of immune cells in vitro. At the same time, Antrodia camphorata is also used in combination with a variety of chemotherapy drugs in research, which can reduce the dosage of chemotherapy drugs and reduce the side effects of chemotherapy. come with side effects, The purpose of this experiment is to culture human lung fibroblasts IMR-90 in vitro to test whether three Antrodia camphorata purified products provided by AMS BioteQ., Ltd. can protect normal human cells undergoing radiotherapy and promote subsequent Recovery, to explore whether Antrodia camphorata, in addition to being combined with chemotherapy, can also become a natural radioprotectant during radiotherapy. In experiments, it was successfully confirmed that one of the three purified Antrodia camphorata was helpful in maintaining cell survival rate, and compared with administration after radiotherapy, the purified Antrodia camphorata YUAN01 was chosen to be administered to IMR-90 cells before radiotherapy. is more effective.