- 學位論文
靜電紡絲法包覆維他命C在藥物釋放及混摻導電高分子在巨噬細胞培養之研究
Preparation of Covered Vitamin C Nanofibers for Drug Delivery and Doping Conductive Polymer Nanofibers for macrophages culture
摘要
本研究利用單軸靜電紡絲製備具有快速溶解特性的電紡絲奈米纖維墊 ，以及含有導電高分子的電紡奈米纖維絲，用於細胞培養以及快速藥物釋放。 以不同的高分子聚碸(PES）與聚苯乙烯(PS）混和二甲基甲醯胺(DMF）作為前驅物溶液，再摻雜不同的導電高分子PEDOT或PANI，以靜電紡絲製備大量奈米纖維；再以聚乙烯吡咯烷酮(PVP)混合酒精作為前驅液，並以維他命C作為目標分子混溶，並以靜電紡絲法製備奈米纖維不織布 (non woven)，接著進行細胞培養及藥物釋放的實驗，通過場發射電子顯微鏡( FE-SEM )、熱重損失分析儀(TGA)、熱機械分析儀(TMA)及水滴角測試儀對奈米纖維特性進行分析，並探討細胞生長與藥物釋放的情形。 實驗結果顯示，藥物釋放的奈米纖維絲線，在經過置於空氣中不同的時間，會因為水氣及其本身的性質獲得收縮的能力，經過長時間收縮的奈米纖維絲會失去其奈米的性質，進而導致溶解的速度降低，進而可以控制其釋放速率。而細胞培養的電紡絲試片，可以發現兩種高分子都具有一定的生物相容性，其中PES可以提供更好的細胞生長空間，而兩者在添加了導電高分子後皆有更為良好的細胞生長能力，其中PEDOT能夠使細胞更為良好的生長能力。
並列摘要
In this study, nanofiber mats with rapid dissolution characteristics and nanofibers containing conductive polymers were prepared by uniaxial electrospinning for cell culture and rapid drug release. Firstly, precursor solution was prepared: the polysulfone (PES) and Polystyrene (PS) dissolved in Dimethylformamide (DMF) and doping conductive polymer poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT) and Polyaniline (PANI) to make sure the concentration of solution in appropriate using single-nozzle electrospinning to produce the nanofibers, and this nanofibers used in macrophages culture. Secondly, precursor solution was prepared: the polyvinylpyrrolidone (PVP) dissolved in ethanol and doping Vitamin C to make sure the concentration of solution in appropriate using single-nozzle electrospinning to produce the nanofibers, and this nanofibers used in drug delivery. The characterized of nanofibers, following instruments were used: Field Emission Scanning Electron Microscope (FE-SEM), Thermogravimetric Analyzer (TGA), Thermal Mechanical Analyzer (TMA), FE-SEM were used to explore the morphology and diameter of nanofibers. Water droplets were used to analyze the properties of nanofibers. Finally, Optical microscope used for macrophages culture in nanofibers.