利用HPLC探討抗氧化乳液的安定性及取代矽靈的配方開發

Using HPLC to Investigate Stability of Antioxidation Lotion and Developing Formula of Substitution Dimethicone

劉鴻澤(Hung-Tse Liu)

指導教授 ： 許世興

朝陽科技大學/理工學院/應用化學系/碩士(2014年)

摘要

乳液是非常普遍的化妝品，為了使乳液在上增加質感，通常會添加矽靈類物質。此成分對皮膚有過敏及發炎的現象，因此本研究目的是開發出不使用矽靈的乳液產品。並添加Q10及維生素E來當作抗氧化成分，建立出簡單快速且準確的HPLC方法來分析這兩種成分。
本研究所建立的HPLC分析方法，層析管柱：HYPERSIL ODS-C18（5μm，4.6 mm × 250 mm），移動相為甲醇：二氯甲烷(70:30)，波長 275 nm偵測Q10及 225 nm偵測維生素E，流速：1.0 ml∕min及注入量為10 μL，在9分鐘內可同時分析這兩種抗氧化劑。
在安定性試驗結果中，檢測含有抗氧化成分乳液在25℃條件下放至三個月，Q10及維生素E含量分別為95.3%及95.1%。在分析方法確效中，Q10的相對標準偏差介於0.32﹪～3.66﹪，回收率為97.11%，線性迴歸R2＝0.9936，偵測極限1.1 ppm，定量極限3.6 ppm；ViE的相對標準偏差介於1.25﹪～4.42﹪，回收率為97.23﹪，線性迴歸R2＝0.9936，偵測極限2.5 ppm，定量極限8.3 ppm。在破壞實驗中，只有加熱、加H2O2及照射UV對主成分不會造成太大的影響含量都有77﹪以上，但是在加強酸與加強鹼中對於Q10及VitE的影響較大且有明顯衰退。

關鍵字

Q10 ； 抗氧化劑 ； 乳液 ； 化妝水 ； 高效液相層析儀 ； 維生素E

並列摘要

Emulsions are very common cosmetics. Dimethicone related substances are usually added to emulsion formula to increase the smooth texture. These substances are hypersensitive and inflammable to skin. Therefore, the purpose of this research is to develop the emulsion product with the smooth texture and without the content of dimethicone. Furthermore, the developed emulsion was added to Q10 and vitamin E as the antioxidants. And, a simple, fast and accurate HPLC method was established to analyze these two ingredients.
The developed HPLC method was performed with the conditions: column (Hypersil ODS-C18; 5μm; 4.6 mm × 250 mm), mobile phase(methanol: dichloromethane = 70:30), detection wavelength 275 nm and 225nm for Q10 and vitamin E, respectively, flow rate 1.0 ml/min, injection amount 10 μL, and the run time was within 9 minutes to simultaneously analyze these two antioxidants. In the stability tests, the formula of antioxidant emulsion showed the satisfied results with the 95.3% contents of Q10 and 95.1% contents of vitamin E in three month of 25℃room temperature condition.
In the results of validation experiment, the relative standard deviation (RSD) of Q10 is ranged from 0.32% to 3.66%, the average recovery was 97.11%, the linear regression coefficient (R2) was 0.9936, and the limits of detection (LOD) and quantification (LOQ) were 1.1 ppm and 3.6 ppm, respectively. The relative standard deviation of vitamin E is ranged from 1.25% to 4.42%, the average recovery was 97.23%, the linear regression coefficient was 0.9936, and the limits of detection and quantification were 2.5 ppm and 8.3 ppm, respectively. In the stress experiments of method validation, the contents of Q10 and vitamin E were quite stable and remained more than 77% during the test of heating, adding strong oxidants and illuminating UV light. Nevertheless, the contents of Q10 and vitamin E were unstable and critically decayed during the test of adding strong acid and base.

並列關鍵字

Antioxidant ； Emulsion ； Lotion ； HPLC ； Q10 ； vitamin E

參考文獻

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