酒粕經乳酸菌醱酵液的生理功能的探討

The function of sake lees after fermented by lactic acid bacteria

鄭家珍(Chia-chen Cheng)

指導教授 ： 顏聰榮

大同大學/工程學院/生物工程學系所/碩士(2009年)

摘要

近年來的研究報告指出，乳酸菌對於人體腸道不適症狀以及急性腸道炎都具有相當程度的改善，而且也具有能調節幼兒腸道菌叢以及能改善免疫系統的效果；在癌症預防上，也證明可以降低癌前病變及腫瘤的機會。故本實驗利用清酒酒粕經不同溶劑萃取後經乳酸菌醱酵，以醱酵液做為美白以及抑制癌細胞生長之研究目標。研究結果顯示酒粕濃度越高、時間越長對於癌細胞抑制效果越強。抑制癌細胞方面，以n-haxane萃取酒粕抑制結腸癌細胞效果較佳，肝癌細胞方面，則以ethyl acetate萃取酒粕有較佳效果，且皆隨酒粕濃度及醱酵時間時間增加而降低存活率。在美白上，對酪胺酸酶在酒粕濃度1 mg/ml以上的抑制差別並不明顯，故選用使用ethyl acetate萃取、醱酵時間72小時、酒粕含量1 mg/ml之醱酵液作為最佳醱酵條件，以Lb. plantanum E38醱酵液處理可降低酪氨酸酶活性至54 %，細胞存活率約為102%且細胞內黑色素降低至54 %，約相當於3.4 μg/ml之黑色素；抑制肝癌細胞與結腸癌細胞方面，會隨酒粕濃度及醱酵時間改變對細胞抑制效果，DPPH清除效果大多也會隨著酒粕濃度降低而減弱。利用HPLC液相層析分析，推測美白有效成分可能是亞麻油酸；而抑制癌細胞部分則可能與亞麻油酸之異構物有關。

關鍵字

功能性 ； 抑制癌細胞 ； 美白 ； 酒粕醱酵液

並列摘要

There are many report have exhibit about Lactic acid bacteria, which can improve intestine discomfortable and Infectious diarrhea, and also improving immunization system. In cancer prevent, it’s already prove that can decrease some types of cancer. In this study, the five lactobacillus strains isolated from local Taiwanese fermented vegetables were used to fermented with different concentrations of sake lees extract (use different solvent extract, 10, 1, 0.1 mg/ml), and the supernatant filtrates were used to keep skin whitening and inhibition cancer cell growth. According to the results, to inhibit Hepatocellular Carcinoma and colon cells growth, cell survivability well decrease when enhancing concentrations of sake lees extract, and the supernatant filtrates from Lb. plantanum E38 could obviously inhibit the mushroom tyrosinase activity of 54 % , and decrease melanin contents of B16-F0 cell line of 54 % , equal to 3.4 μg/ml melanin. DPPH radical scavenging effect well decrease to reduce concentrations of sake lees extract for the most part. The tyrosinase inhibitory compounds contained in supernatant filtrates identify as linoleic acid using HPLC. The cancer inhibitor maybe inoleic acid isomers, presently have many report about inoleic acid isomers can anti-inflammatory and anticarcinogenic properties. Therefore, the fermentation products from sake lees by the lactobacilli will be very useful for application in the cosmetic and anticarcinogenic medicine.

並列關鍵字

Functionality ； Whiting ； Anti-cancer proliferation ； Sake-lees

參考文獻

1.許譽繼. 2006. Sake brewing and analysis of sake flavors. 清酒的釀造及其香味之分析研究. 41-42.

2.de Roos, B. Z., S. E.Gilliland, and C. V. Maxwell. 1996. Hypocholesterolemic action of Lactobacillus acidophilus ATCC 43121 and calcium in swine with hypercholesterolemia induced by diet. J. Dairy Sci.79:2121-8.

3.kociubinski, G, P. Perez, M. Anon, and G. De Antoni. 1996. Amethod of screening of highly inhibitory lactic acid bacteria. J.Food Prot. 59:1-8

4.Majamaa, H., E. Isolauri, M. saxelin and T. Vesikari. 1995. Lactic acid bacteria in the treatment of acute rotavirus gastroenteritis. J. Pediatric Gastroenterol. and Nutrition. 20：333-338.

5.Motlagh, A. M., M. C. Johnson and B. Ray. 1991. Viability loss of foodborne pathogens by tarter cultures metabolites. J. Food Protect.873-878