"Wu Zhu Yu Tang" is one of the classic formulae of Traditional Chinese Medicine, which originated from the masterpiece "Shanghan Zabing Lun" by "Zhang Zhong-jing" (Eastern Han Dynasty). There were lots of research and discussion of this formula from the past history. We review these important works of herbal medicine and those ancient Doctors' research, trying to realize the mechanism of Chinese medicine and six-meridian positioning of this formula. Besides, we also review many modern articles and research from the medical databases, trying to explore the pharmacological mechanism and toxicity, as well as the timing of clinical use. In addition, we analyze the data of this formula used in our hospital from November 2015 to June 2017, hoping to expand the clinical application of "Wu Zhu Yu Tang" and to find out its clinical guideline.