This topic discusses the applicative strategy and management of Disk Quota at LINUX and Windows Operating Systems. We suggest an practical strategy and architecture for Small Business and School Laboratory Group We also compare the differences about Disk Quota between LINUX's (based on Red Hat and SUSE distributions) and Windows operating systems. We advance the best applicative structure for Disk Quota between these two systems constituted with Windows's dynamic disk or LINUX's LVM and security access control settings in order to get more flexibility and availability for maintenances and managements.