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P53 Mutation in Neuroepithelial Cell Tumor of Brain



Parallel abstracts

Tumors of the central nervous system account for approximately 1.84% of all human cancers in Taiwan. Most of adult primary brain tumors are gliomas, of which astrocytomas are the most common ones. These tumors are progressive, tend to recur following treatment, and are usually fatal. Recent studies have shown that cancers progress as a result of several sequential genetic events, occurring stepwise over time, and may involve either activation of oncogenes or inactivation of tumor suppressor genes. Tumor suppressor genes, especially p53, are now known to play an important role in the development of a wide variety of human cancers. In the study, polymerase chain reaction and single-strand conformation polymorphism (PCR-SSCP) analysis was used to investigate the mutation of p53 gene in 116 surgically removed primary brain tumor tissues which include 62 astrocytomas, 23 oligodendrogliomas, 11 ependymomas and 20 primitive neuroepithelial tumors (PNET). 14.7% (17/116) of the brain tumors had p53 mutation and were found to be localized in exons 5, 7, and 8. Mutations do not follow a random distribution among different subtypes, but occurred in 17.3% (9/52) of grade Ⅱ and Ⅲ of astrocytomas, 22.2% (2/9) of grade Ⅲ oligodendrogliomas and 25% of PNETs. All p53 mutations were single nucleotide change, mostly missense mutations (17 events). The majority of mutations identified in this study were G:C to A:T or C:G to T:A transitions (60%, 12 of 20) and occurred frequently (60%, 12 of 20) at sites of CpG dinucleotides, which are known to be hot spots for mutations in the p53 gene in many other types of human cancers. These results suggest that the inactivation of p53 gene plays an important role in the development of brain tumor.

Parallel keywords

brain tumor p53 gene