- 期刊
大白鼠腮腺第 I 型去氧核醣核酸酶之免疫組織化學定位
Immunohistochemical Localization of Deoxyribonuclease I in Rat Parotid Gland
摘要
背景：去氧核醣核酸酶為腮腺所必的消化酵素之一。近年來隨著分子生物技術及生化科學的進步，吾人對去氧核醣核酸酶在生命現象中所扮演的角色，有了更進一步的認識，除了已知的消化功能外，其與許多癌症和細胞凋亡(apoptosis)都存在著某種程度的關聯。 方法：本研究利用純化出之抗體，以免疫組織化學染色法及免疫電子顯微鏡技術定位大白鼠第 I 型去氧核醣核酸酶(deoxyribonuclease I, DNase I)於腮腺及胰臟組織的分佈情形。 結果：大白鼠DNase I 主要存在於腮腺腺胞(acinar cells)的分泌顆粒中，而異於其他哺乳動物的是大白鼠胰臟中並無DNase I 的存在。 結論：由本研究結果得知腮腺在消化系統中，因動物種類的不同可能扮演著不同功能的角色。同時，因了解DNase I 在腮腺的分佈情形，使有關腮腺細胞生物學方面的知識提昇很多，對於進一步的研究應當有所幫助。
並列摘要
Background: Deoxyribonuclease (DNase) is one of the digestive enzymes secreted by the parotid gland. Recent progresses in molecular biology and biochemistry have enabled us to learn more about the function of DNase in cells. In addition to its well-known digestive function, DNase was found to be closely related to some cancers and apoptosis.] Methods: We use immunohistochemical techniques with purified antibody to study the distribution of DNase I in rat pancreas and parotid gland. Results: Rat DNase I is localized in secretary ganules of parotid gland but not found in pancreas. Conclusions: Our results suggest that the role in digestive function of parotid gland may differ among different species and localization of DNase I may help us to the further study of parotid gland in cell biology.