In this paper an automatic optical measurement system for the fracture conditions of anisotropic conductive film(ACF) is presented. On the process of searching, analyzing, and recognizing of ACF-LCD images, the measurements include the quality verification and quantity calculation of ACF. We used different methods to determine the fracture factors and the ACF area for each type. The improving method of the template matching, and largely decreasing the time of checking the image for pattern matching was verified in the experimental results of this imaging system.